Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof
A technology for root-knot nematode disease and compound fertilizer, which is applied in the preparation of organic fertilizer, biological organic part treatment, organic fertilizer and other directions, can solve the problems of peanut root-knot nematode disease that is not targeted well and cannot provide organic nutrition for peanuts.
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[0023] The preparation method of the special organic-inorganic compound fertilizer for peanuts for biologically controlling root-knot nematode disease of the present invention, the steps are as follows:
[0024] (1) preparation of actinomycetes fermented liquid: including actinomycetes slanted seed culture, connect slanted seeds to liquid medium to obtain seed liquid, and then cultivate the seed liquid in liquid medium to obtain actinomycetes fermentation liquid, bacteria The viability index is greater than 1*109 per milliliter.
[0025] (2) Preparation of decomposed organic matter: in parts by mass, 60-70 parts of xylitol residue, 13-20 parts of smoke residue, 10-15 parts of calcium-magnesium-phosphate fertilizer, 3-5 parts of ferrous sulfate, 1 ~2 parts are mixed well. The moisture content of the mixture is adjusted to be 60-70 wt%, the C / N ratio is 23-30, and the pH is 7-7.5.
[0026] The above mixture is piled into a trapezoidal pile with a height of 1.5-1.8 meters, the ...
specific Embodiment approach
[0042] The present invention will be further described below in conjunction with the embodiments, but not limited thereto. The description of the raw materials is as follows:
[0043] In the embodiment, the moisture content of the xylose residue is 50wt%, and the organic matter content is 60wt%, which is the waste residue produced during the production of xylose by Shandong Longli Biotechnology Co., Ltd. using corn cob as raw material; the organic matter content of the smoke residue is 53.21wt%, which is Henan China Tobacco. Tobacco leaf residue discarded in the production process of an industrial company. The calcium magnesium phosphate fertilizer contains 16wt% phosphorus pentoxide, produced by Xingyi Chemical Fertilizer Factory in Kunyang, Yunnan. The microbial fermentation inoculum is an EM compound inoculum, produced by Jiangxi Tianyi Biotechnology Development Co., Ltd.
[0044] The actinomycetes were Bacillus subtilis (Bacillus subtilis), the strain catalog number 1.93...
Embodiment 1
[0047] (1) Preparation of actinomycete fermentation broth: slanted seeds: medium formula: NaCl 19.5 g, MgCl 25.9 g, Na 2SO4 3.3 g, CaCl 21.9 g, peptone 5.0 g, yeast extract 1.0 g, agar 16 g, water 1000 ml, pH 7.5. Sterilize under a pressure of 0.11 MPa for 30 minutes, inoculate the original actinomycetes Bacillus subtilis 1.933 under sterile conditions after making the inclined plane, and place it in a constant temperature incubator at 28°C for 2 days.
[0048] Fermentation broth: The medium formula is glucose 15.0g, mannitol 5.0g, peptone 10.0g, yeast extract 1.0g, beef extract 2.0g, NaCl 20.0g, CaCO 3 1 g, 1000 ml water, pH 7.5. The slanted seeds were connected to the liquid medium and cultured with shaking at 200 reciprocation / min for 3 days at 28° C. to obtain the seed liquid. Then, the inoculum amount of 1 wt% was shaken and cultured in the liquid medium for 8 days to obtain the actinomycete fermentation broth. The bacterial viability index is 1.16*10 9 pcs / ml
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