Method for preparing kit for testing hemolytic disease of newborn
A detection kit, a technique for neonatal hemolysis, applied in the field of blood transfusion medicine, can solve the problems of easy missed detection, cumbersome operation, wrong detection, etc., and achieve the effect of strong buffering ability
Active Publication Date: 2013-01-09
JIANGYIN LIBO MEDICINE BIOTECH
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- Description
- Claims
- Application Information
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Problems solved by technology
[0024] The detection methods used include enzyme test method, polybrene method, microgel column method, antihuman globulin technology and other methods. The measured potency is higher than the actual potency, etc.
So far, there is no specific kit production at home and abroad that is easy to operate, low in cost, and dedicated to the detection of hemolytic disease of newborns
Method used
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Embodiment 1
[0062] Step 1, preparation of gel suspension medium
[0063] Described gel suspending medium formula is as follows:
[0064] Methylparaben (5.5-6.5)×10 -4 g / ml
[0065] Propylparaben (1.0-1.5)×10 -4 g / ml
[0066] Glycine (1.6-1.9)×10 -2 g / ml
[0067] Sodium chloride (1.7-1.8)×10 -3 g / ml
[0068] Potassium dihydrogen phosphate (2.1-2.4)×10 -4 g / ml
[0069] Disodium hydrogen phosphate (4.6-4.8)×10 -4 g / ml
[0070] Bovine serum albumin ≤2%,
[0071] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.
[0072] Step 2. Preparation of the gel
[0073] Polyacrylamide dextran gel is selected, and the particle size is 30-60 nanometers. Soak in the gel suspension medium prepared in step 1, then wash with the gel suspension medium for 3-5 times, remove the broken fragments of the gel and the aggregated gel particles, and collect the applicable gelatin powder with uniform particle size and complete spherical shape. gel.
[0074] Step 3. Antib...
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Abstract
The invention relates to a method for preparing kit for testing the hemolytic disease of the newborn (HDN), which is characterized in that the kit comprises the following four microcolumn gel cassettes: an HDN antepartum prediction cassette I, an HDN antepartum prediction cassette II, an HDN postpartum test cassette I and an HDN postpartum test cassette II, and the four cassettes respectively have 6 microcolumn gel tubes. The method comprises the following technological processes: confecting gel suspension media, preparing gel, selecting antibodies and suspending and subpackaging gel. The formula of the gel suspension media includes (5.5-6.5)*10-4g / ml of methyl-p-hydroxybenzoate, (1.0-1.5)*10-4g / ml of propyl p-hydroxybenzoate, (1.6-1.9)*10-2g / ml of glycine, (1.7-1.8)*10-3g / ml of sodium chloride, (2.1-2.4)*10-4g / ml of potassium dihydrogen phosphate, (4.6-4.8)*10-4g / ml of disodium hydrogen phosphate and not more than 2% of bovine serum albumin. The above reagents are dissolved with distilled water and the pH value is regulated between 6.6 and 6.8. The kit prepared by the method of the invention has high sensitivity, good specificity and stable quality, and is specially used for testing HDN.
Description
(1) Technical field [0001] The invention relates to a blood type serology detection kit for blood type identification and application thereof. More specifically, the present invention relates to the composition, preparation method and application of a detection kit for hemolytic disease of newborns, and relates to the field of transfusion medicine. (2) Background technology [0002] Hemolytic disease of the newborn (abbreviated as HDN) refers to the incompatibility of maternal and infant blood types. The red blood cells of the fetus and newborn are coated by the mother's IgG blood group antibody and are destroyed in the reticuloendothelial system of the infant. [0003] The cause of this disease is maternal and fetal blood group incompatibility. Half of the blood type genes of the fetus come from the mother and the other half from the father. When the dominant antigen inherited by the fetus from the father is just lacking in the mother, the antigen enters the mother through...
Claims
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IPC IPC(8): G01N33/531G01N33/80G01N33/577
Inventor 陈玉平钱国强朱庆平
Owner JIANGYIN LIBO MEDICINE BIOTECH