Fat-soluble biological composite fermentation accelerator and application thereof

A technology of compound fermentation and accelerator, applied in the direction of fermentation, etc., can solve the problems of cell division, value-added, affecting enzyme content and activity, high energy consumption and other problems of different strains of bacteria, so as to promote division and proliferation, increase content and activity , improve the effect of economic

Inactive Publication Date: 2010-06-16
济南沃德生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The single soybean oil or single vegetable oil currently used cannot fully satisfy the division and value-added of different strains of bacteria, directly affects the content and activity of en...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Fat-Soluble Biological Composite Fermentation Accelerator

[0031] 1. Take raw material according to following composition and content: (1) lipid component is methyl oleate 0.5g / L; (2) vitamin component is vitamin A 0.5mg / L; (3) amino acid component is L-valamine acid 0.1g / L; (4) 4 kinds of growth factors include phosphoinositides, choline, uracil and thymidine, the content of each growth factor is 0.01g / L; these raw materials are mixed evenly after being treated by a micronizer, and added Sesame oil is fixed to volume, and the sesame oil is the balance of the above four ingredients.

[0032] 2. Take the raw materials according to the following components and contents: (1) The lipid components are methyl linoleate 0.2g / L, methyl palmitate 0.1g / L, methyl arachidonic acid 0.1g / L and twenty Dicosahexaenoic acid methyl ester 0.1g / L; (2) The vitamin composition is vitamin A 0.1mg / L, vitamin E 0.2mg / L and vitamin D 0.2mg / L; (3) The amino acid composition is L-...

Embodiment 2

[0038] The fat-soluble biocomposite fermentation accelerator of the present invention is applied to lipase fermentation.

[0039] The strain Yarrowia lipolytica JK was activated once by PDA solid medium and twice by PDA liquid medium, inoculated into PDA liquid medium, and cultured at 28°C for 12 hours.

[0040] PDA medium preparation: 200g of potatoes, washed, peeled and chopped, add 1000ml of water to boil for half an hour, filter through gauze, add 20g of glucose, sterilize at 115°C for 30 minutes, add 15g of agar to the solid medium.

[0041] The composition and proportion of the fermentation medium are (w / v): 6% soybean powder, 4% soybean oil, 2% (v / v) fat-soluble biocomposite fermentation accelerator, (NH 4 ) 2 SO 4 0.2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, pH 6.5.

[0042] The components and proportions of the control fermentation medium are (w / v): soybean powder 6%, soybean oil 4%, (NH 4 ) 2 SO 4 0.2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, pH 6.5.

...

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PUM

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Abstract

The invention discloses a fat-soluble biological composite fermentation accelerator, which belongs to the technical field of biological fermentation. The fermentation accelerator consists of a lipoid component, a vitamin component, an amino acid component, four growth factors and sesame oil. The fat-soluble biological composite fermentation accelerator can remarkably improve the permeability of cell membranes, supplement multiple nutrient components required for maintaining the growth of microbe, promote the division and propagation of microbial cells, obviously improve the content of living bacteria in fermentation liquor, protect the cells from being damaged by free radicals, and delay ageing and autolysis of the cells so as to prolong the time of synthesizing fermented metabolic products and fulfill the aim of increasing the yield of a target metabolic product.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a fat-soluble biological composite fermentation accelerator and its application. Background technique [0002] The modern definition of fermentation is a reaction process in which a large number of specialized metabolites are produced and accumulated through the growth, culture and chemical changes of microorganisms (or animal and plant cells). In industrial production, all industrial production that relies on the life activities of microorganisms is generally called "fermentation". The level of microbial metabolites is controlled by a variety of regulatory mechanisms, so the synthesis of microbial end products is under normal It will not exceed the amount needed for its own growth. [0003] In the past hundred years, with the advancement of science and technology, fermentation technology has undergone epoch-making changes. It has entered the stage of using the o...

Claims

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Application Information

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IPC IPC(8): C12P1/00
Inventor 程寿惠颜峰
Owner 济南沃德生物技术有限公司
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