Microfluidic chip system integrating cell operation and detection

A microfluidic chip and microchip technology, applied in the direction of material resistance, etc., can solve the problem of difficult coordination between efficient cell capture and high-sensitivity detection of impedance, the inability to realize the integration and miniaturization of the DEP analysis system, and the inability to achieve high-sensitivity detection and other issues to achieve the effects of enhanced stability, improved detection sensitivity, and miniaturization

Inactive Publication Date: 2010-06-16
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main disadvantage of this system is that it can only detect the system impedance and cannot realize the manipulation of cells. At the same time, the detection limit is also very high. In the case of few cells, no effective signal can be detected. In the early stage of the disease, the markers in the sample can be analyzed. disease-causing cells are usually few, therefore, the system does not meet the requirements of highly sensitive detection
The main disadvantages of this system are: large-sc

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  • Microfluidic chip system integrating cell operation and detection
  • Microfluidic chip system integrating cell operation and detection
  • Microfluidic chip system integrating cell operation and detection

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Embodiment 1

[0024] Such as Figure 1~4 As shown, a microfluidic chip system integrating cell manipulation and detection mainly includes an AC signal power supply 2 , a microchip 4 , an integrated circuit control unit 3 and a computer 1 . The AC signal power supply 2 is connected with the power connector 5 of the integrated circuit control unit 3 through wires, the microchip 4 is connected with the chip interface 7 of the integrated circuit control unit through its pins, and the integrated circuit control unit 3 is connected with the computer 1 through a data line. A complete and stable integrated dielectrophoresis (DEP) analysis microsystem is formed.

[0025] The AC signal power supply 2 is an adjustable AC signal generator, which is connected to the 220V mains through the power line, and its output signal voltage is 0-10V, and the frequency is 100Hz-10MHz, and the signal output terminal is connected to the integrated circuit control unit 3 through the cable. The power connector 5 is conn...

Embodiment 2

[0030] A microfluidic chip system for integrated cell manipulation and detection, the same as in Embodiment 1, wherein: the integrated circuit control unit 3 is an external circuit control unit, which is an integrated micro-control circuit board, and the length of the circuit board is 10 cm and the width is 10 cm. It is 8cm. Polydimethylsiloxane (PDMS) cover slip with tubing The tubing has a length of 10 mm, a circular cross-sectional shape, and an outer diameter of 150 μm. The manipulation unit electrodes 15 are arrayed titanium-gold micro-counter electrodes, 15 pairs in total. The length of each electrode is 50 μm, the cross-sectional shape of the electrode is rectangular, the width is 30 μm, the length is 50 μm, the distance between each pair of electrodes is 30 μm, and the distance between two adjacent pairs of electrodes is 20 μm. The electrodes 16 of the impedance detection unit are interdigitated titanium gold microarray electrodes, and there are 25 pairs in total. Th...

Embodiment 3

[0032] A microfluidic chip system for integrated cell manipulation and detection, the same as in Embodiment 1, wherein: the integrated circuit control unit 3 is an external circuit control unit, which is an integrated micro control circuit board, and the length of the circuit board is 15 cm and the width is 15 cm. It is 10cm. Polydimethylsiloxane (PDMS) coverslip with tubing The tubing has a length of 10 mm, a circular cross-sectional shape, and an outer diameter of 300 μm. The manipulation unit electrodes 15 are arrayed titanium-gold micro-counter electrodes, 25 pairs in total. The length of each electrode is 70 μm, the cross-sectional shape of the electrode is rectangular, the width is 40 μm, the length is 70 μm, the distance between each pair of electrodes is 50 μm, and the distance between two adjacent pairs of electrodes is 30 μm. The electrodes 16 of the impedance detection unit are interdigitated titanium gold microarray electrodes, totally 50 pairs. The length of eac...

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Abstract

The invention provides a microfluidic chip system integrating cell operation and detection and relates to a device for cell manipulation and detection in early diagnosis of diseases. The microfluidic chip system mainly comprises an alternating current signal power supply, a microchip, an integrated circuit control unit and a computer. The microfluidic chip system is characterized in that: the microchip is formed by bonding polydimethylsiloxane cover glass and a glass substrate, wherein the polydimethylsiloxane cover glass is provided with pipes; the glass substrate is deposited with a cell operation unit electrode and an impedance detection unit electrode; and the integrated circuit control unit is an external circuit control unit, namely, an integrated micro-control circuit board. The microfluidic chip system integrating the cell operation and detection is characterized by high detection sensitivity, good stability, flexible and convenient operation, system micromation, excellent portability, and the like, can be widely applied to the analysis and detection of cells and the early diagnosis of diseases, particularly the acquisition and detection of cancer cells and the early diagnosis of a cancer. The system also can be widely applied to food safety testing, environmental monitoring, medicaments/genes screening, and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical sample analysis, and in particular relates to a device for manipulating and detecting cells in early disease diagnosis. Background technique [0002] With the advancement of science and technology and the improvement of people's living standards, people's expectations for health are getting higher and higher. They expect early diagnosis of diseases, especially cancer, and require timely and effective treatment. The analysis and detection of biochemical samples, such as proteins, DNA, and cells, is one of the methods to achieve early diagnosis of diseases. It can help doctors to detect the disease as early as possible and take effective treatment measures, which has a very important role and significance in improving people's health. Therefore, the analysis and detection of biochemical samples, especially the manipulation and detection of cells, has attracted more and more attention from scien...

Claims

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Application Information

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IPC IPC(8): G01N27/06G01N27/07
Inventor 徐溢吴永杰刘海涛彭金兰曹强温志渝
Owner CHONGQING UNIV
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