Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol

A technology for kitchen waste and fuel ethanol, which is applied in the directions of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of raw materials that cannot be hydrolyzed, lack of glucose, etc., achieves high utilization, and simplifies production processes and equipment. simple effect

Inactive Publication Date: 2010-06-30
TAIZHOU VOCATIONAL & TECHN COLLEGE
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Problems solved by technology

[0014] Technical problem: The present invention mainly provides a new method for producing fuel ethanol by co-fermenting food waste raw materials with mixed bacteria, which solves the related technical problems of producing fuel ethanol through mixed bacteria fermentation of food waste, so as to improve the utilization rate of garbage raw materials and reduce fuel consumption. Ethanol production cost, simplified fermentation production process
[0016] The present invention has the following advantages: the process of co-fermenting the bacterial strains with both amylase and cellulase activities and Saccharomyces cerevisiae is adopted, which avoids the inability of hydrolysis of raw materials due to enzyme inactivation in the whole fermentation process, and the glucose in the fermentation liquid Lack of ethanol fermentation can not complete the problem; no need to add any catalyst and nutrients in the fermentation process, the utilization of macromolecular organic matter such as starch and cellulose in garbage is high, and the components of fermentation residue are simple, which can be directly used as single-cell protein Use of feed additives

Method used

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  • Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol
  • Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol
  • Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol

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Embodiment Construction

[0020] Isolation and identification of bacterial species

[0021] A number of distiller's grains and commercially available koji samples were collected, and different types of distiller's grains and koji medicine samples were enriched and cultivated. After dilution, the colonies isolated by the plate dilution method were inoculated into inclined test tubes, and the preliminary morphological identification was carried out.

[0022] Rinse the surface of the fungal slant test tube culture with normal saline, and perform gradient dilution on the obtained cell suspension. Select a suitable gradient dilution solution and apply it to the surface of the cellulose-Congo red medium and the starch-inorganic salt medium for primary screening. The strains that can produce both cellulase and amylase obtained from the primary screening were re-screened with the enzyme-producing medium, and a strain with both cellulase activity and amylase activity was screened out.

[0023] Streak inoculate...

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Abstract

The invention relates to a new method of co-fermenting kitchen waste raw materials with mixed bacteria for producing fuel ethanol, which is characterized by comprising the following steps: 1, separating and screening a rhizopus oryzae strain which simultaneously has amylase activity and cellulase activity; and 2, taking the kitchen waste as a raw material only, and mixing the rhizopus oryzae and saccharomyces cerevisiae in a certain proportion for co-fermenting to produce the fuel ethanol. The method of the invention avoids the problems that in the process of fermenting the kitchen waste for producing the fuel ethanol, the raw material can not hydrolyze because of enzymic inactivation, glucose is lacked in the fermentation liquor, and ethanol fermentation can not be completely carried out. No catalyst and nutrient need to be added in the fermentation process, the availability of large molecule organic substances of starch, cellulose and the like in the waste is high, thus the invention reduces the production cost of ethanol by the kitchen waste, has the advantages of energy saving, simple required equipment and low investment cost, and is a new way with good practical value for recycling the kitchen waste.

Description

technical field [0001] The invention belongs to the field of renewable energy production, in particular to a method for fermenting and producing fuel ethanol from food waste as raw material Background technique [0002] Food waste refers to food processing leftovers (kitchen waste) and food residues (swill) generated in catering operations and residents’ lives. Food waste has the characteristics of high moisture content and high organic matter content, which is not suitable for traditional incineration and landfill methods; and because of its high salt content, it is greatly restricted for composting raw materials. Therefore, how to scientifically and rationally dispose and recycle kitchen waste has become an urgent problem that deserves people's attention [1, 2, 3]. [0003] Ethanol is the main component of alcohol fuel and one of the clean fuels that cars can use. At present, most of the fuel ethanol produced at home and abroad uses grain as raw material, which consumes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/06C12R1/865C12R1/845
CPCY02E50/17Y02E50/10
Inventor 张昕欣奚立民柯中炉于红艳曹树勇闫启东
Owner TAIZHOU VOCATIONAL & TECHN COLLEGE
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