Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol
A technology for kitchen waste and fuel ethanol, which is applied in the directions of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of raw materials that cannot be hydrolyzed, lack of glucose, etc., achieves high utilization, and simplifies production processes and equipment. simple effect
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[0020] Isolation and identification of bacterial species
[0021] A number of distiller's grains and commercially available koji samples were collected, and different types of distiller's grains and koji medicine samples were enriched and cultivated. After dilution, the colonies isolated by the plate dilution method were inoculated into inclined test tubes, and the preliminary morphological identification was carried out.
[0022] Rinse the surface of the fungal slant test tube culture with normal saline, and perform gradient dilution on the obtained cell suspension. Select a suitable gradient dilution solution and apply it to the surface of the cellulose-Congo red medium and the starch-inorganic salt medium for primary screening. The strains that can produce both cellulase and amylase obtained from the primary screening were re-screened with the enzyme-producing medium, and a strain with both cellulase activity and amylase activity was screened out.
[0023] Streak inoculate...
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