Luminescent and magnetic nano material-based method for detecting carcinoembryonic antigen

A magnetic nano, fluorescent nano technology, applied in fluorescence/phosphorescence, analytical materials, material excitation analysis, etc., can solve the problems of poor photostability, short luminescence time, long emission spectrum, etc., to achieve simplified detection methods and high sensitivity , The effect of covalent binding and stabilization

Inactive Publication Date: 2010-07-14
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they have many limitations in terms of optical performance and photochemical stability, such as short luminescence time, poor photostability, short excitation spectrum and long emission spectrum.

Method used

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  • Luminescent and magnetic nano material-based method for detecting carcinoembryonic antigen
  • Luminescent and magnetic nano material-based method for detecting carcinoembryonic antigen
  • Luminescent and magnetic nano material-based method for detecting carcinoembryonic antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Fe 3 o 4 / SiO 2 Preparation of core-shell nanoparticles:

[0041] 1. Weigh 1.08g of FeCl 3 ·6H 2 O and 0.555g FeSO 4 ·7H 2 O, dissolved in 20ml ultrapure water, vacuumed for 20min, N 2 Saturation for 20 minutes; add excess ammonia water to it to obtain a black precipitate, combined with magnetic separation, wash with ultrapure water several times.

[0042] 2. Mix 7 mg of the product from step 1 with 2.3 mL of absolute ethanol, 2.5 mL of deionized water and 0.7 mg of sodium oleate, stir with a magnet and add 10 μL of GMPS.

[0043] 3. Sonicate the mixture in step 2 for 15 minutes, add 100 μL of TEOS, stir for 1 hour, add 10.2 mL of 28% ammonia water dropwise, and stir for 20 hours to obtain Fe 3 o 4 / SiO 2 Core-shell nanoparticles, combined with magnetic separation, were washed several times with ultrapure water.

[0044] (2) Preparation of CdTe quantum dots:

[0045] 1. Vacuumize 40ml of buffer solution containing 15mM boric acid and 15mM sodium citrate ...

Embodiment 2

[0062] Detect the content of CEA in the patient's serum:

[0063] 1. Take 1ml of serum and mix with 100μl of "Fe 3 o 4 / SiO 2 - The first antibody" was mixed and reacted at room temperature for 30 minutes;

[0064] 2. Combining with magnetic separation, wash each microcentrifuge tube sample in step 1 with PBS buffer solution of pH 7.4 twice, and then dissolve to 1.0ml, then add 200μl of "CdTe-secondary antibody" complex respectively, and store at room temperature React for 30 minutes;

[0065] 3. Combined with magnetic separation, carefully wash twice with PBS buffer solution with pH 7.4, use the buffer solution to make up to 0.5ml, and measure fluorescence;

[0066] 4. Bring the obtained results into the working equation Y=A(X-B), calculate the content of CEA in the serum, and compare the results with the results of immunofluorescence detection, see Table 1.

[0067] Table 1 Detection method of the present invention and immunofluorescence detection result contrast

[00...

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Abstract

The invention relates to a luminescent and magnetic nano material-based method for detecting carcinoembryonic antigen (CEA), which comprises the following steps: (1) preparing a FE3O4/SiO2 nuclear shell-first antibody compound; (2) preparing a CdTe-second antibody compound; (3) drawing a 'fluorescence intensity-CEA concentration' standard working curve and calculating a working equation; and (4) detecting a fluorescence intensity value, and obtaining the content of the CEA of the patient through the working equation. The detection method has the advantages of simpleness and high sensitivity, and can easily detect the illness state of patients in the early stage of cancer.

Description

technical field [0001] The invention belongs to the field of detection methods of carcinoembryonic antigen (CEA), in particular to a detection method of carcinoembryonic antigen based on fluorescent nanoluminescence and magnetic nanomaterials. Background technique [0002] Carcinoembryonic antigen (CEA) is a glycoprotein in fetal intestine, liver, pancreas and other tissues, with a molecular weight of 150-2000 kb. It is very low in normal human serum, and the amount increases after canceration occurs. It is currently known that in colon cancer, rectal cancer and other malignant tumors derived from endoderm, such as esophagus, stomach, liver, pancreas, etc., it also increases when it becomes cancerous. The index of CEA in normal people is less than 5ng / ml, but the CEA of smokers or some people is detected to be higher than 5ng / ml, which is also a normal level. Therefore, CEA is not a cancer-specific antigen, but should be considered as a cancer-associated antigen. Determina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/574G01N21/64
Inventor 周兴平李靖
Owner DONGHUA UNIV
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