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Method for separating and purifying coptisine monomers

A technology for separation and purification of coptisine, which is applied in the field of separation and purification of coptisine monomer, can solve problems such as inability to strengthen the determination of coptisine content, no coptisine, lack of high purity, etc., achieve good separation effect, easy to scale up production, and easy The effect of product quality

Inactive Publication Date: 2012-03-07
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to solve the above-mentioned problems such as the absence of high-purity coptisine separation and preparation method in the above-mentioned prior art, the lack of high-purity coptisine monomer compound, and the inability to strengthen the quality control of coptis and its preparations through the content determination of coptisine , providing a method for separation and purification of coptisine monomer

Method used

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  • Method for separating and purifying coptisine monomers
  • Method for separating and purifying coptisine monomers
  • Method for separating and purifying coptisine monomers

Examples

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Comparison scheme
Effect test

Embodiment 1

[0047] The separation and purification method of coptisine monomer of the present embodiment comprises the following main steps:

[0048] (1), extraction:

[0049] Take 1 kg of Coptidis Rhizoma, crush it into a coarse powder, add 10 L of 85% ethanol solution by volume, reflux extraction 3 times, each time for 2 hours, filter, collect, and combine the filtrates (the dregs are evaporated to dryness to recover ethanol).

[0050] (2), concentration:

[0051] The filtrate collected and combined in step (1) is recovered with ethanol, concentrated to a relative density of 1.10g / mL (measured at about 90°C), left to stand for crystallization for 8 hours, filtered, and the precipitate is removed (the crystal in the precipitate is Berberis Alkaline, separately purified to obtain the berberine product), coptisine is mainly retained in the filtrate, and the obtained filtrate (about 1.5 L) is subjected to the next step of extraction treatment.

[0052] (3), extraction:

[0053] Add ammon...

Embodiment 2

[0068] The separation and purification method of coptisine monomer of the present embodiment comprises the following major steps:

[0069] (1), extraction:

[0070] Take 10 kg of Coptidis Rhizoma, crush it into a coarse powder, add 80 L of 95% ethanol solution by volume, reflux extraction 4 times, each time for 3 hours, filter, collect and combine the filtrates (the dregs are evaporated to dryness to recover ethanol).

[0071] (2), concentration:

[0072] The filtrate collected and combined in step (1) is recovered to ethanol, concentrated to a relative density of 1.05g / mL (measured at about 90°C), left to stand for crystallization for 12 hours, filtered, and the precipitate is removed (the crystal in the precipitate is Berberis Alkaline, separately purified and processed to obtain the berberine product), Coptisine is mainly retained in the filtrate, and the obtained filtrate (about 30L) is subjected to the next step of extraction treatment.

[0073] (3), extraction:

[007...

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Abstract

The invention discloses a method for separating and purifying coptisine monomers, comprising the steps of extracting, concentrating, leaching, dissolving and filtering, performing preparative high performance liquid chromatography and separating, recycling products and the like. The method of the invention has simple and convenient operation, short production cycle, high separating efficiency, stable process and low cost, and can realize preparing great amount of the high-purity coptisine monomers by separation; and the obtained coptisine monomers have high purity, and can be used as comparison products in assaying.

Description

technical field [0001] The invention relates to the technical field of methods for isolating compound monomers from plants, in particular to a method for separating and purifying coptisine monomers. Background technique [0002] Coptis coptidis (Rhizoma coptidis) is the dry rhizome of Coptis chinensis Franch., Coptis deltoidea C.Y.Cheng et Hsiao or Coptis teeta Wall. "Yalian", "Yunlian". Coptis chinensis is bitter and cold in nature, and it has the effects of clearing away heat and dampness, purging fire and detoxifying through the heart, spleen, stomach, liver, gallbladder and large intestine meridians. For damp heat and fullness, vomiting and acid regurgitation, diarrhea, jaundice, high fever and coma, hyperactivity of heart fire, upset and insomnia, red eyes, toothache, thirst, carbuncle and furuncle; external treatment of eczema, eczema, ear canal Pus. [0003] Coptisine is a characteristic chemical component of Coptis chinensis, and it is also the most active proberb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/22
Inventor 文焕松郭建华刘丁夏柯
Owner CHENGDU PUSH BIOLOGICAL TECH
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