Method for separating and purifying xylose and arabinose from hemicellulose acid hydrolysis liquid

A technology of hemicellulose and arabinose, applied in the field of simulated moving bed chromatographic separation, can solve the problems such as difficult to effectively control the process, affect industrial production, and uneconomical separation process, achieve less mobile phase consumption, realize continuous operation, The effect of improving the utilization rate of raw materials

Inactive Publication Date: 2012-07-25
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of membrane technology, the cut-off pore size of the membrane is not absolute, but the average value of a range. In addition, the concentration of the separated sugar solution is high, so a longer column membrane is often required to achieve a higher degree of separation. length, making the separation process uneconomical
Both the enzyme separation method and the microbial fermentation method remove one or several components in the sugar liquid, the process is difficult to effectively control, and the use cost is high, which affects industrial production

Method used

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  • Method for separating and purifying xylose and arabinose from hemicellulose acid hydrolysis liquid
  • Method for separating and purifying xylose and arabinose from hemicellulose acid hydrolysis liquid
  • Method for separating and purifying xylose and arabinose from hemicellulose acid hydrolysis liquid

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Experimental program
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Embodiment 1

[0026] A method for separating and purifying xylose and arabinose from hemicellulose acid hydrolyzate, the steps are as follows:

[0027] (1) The hemicellulose hydrolyzate directly obtained by acid hydrolysis is subjected to electrodialysis, membrane filtration and concentrated pretreatment, and the measured concentration is 40%. The high-purity water is deoxygenated and filtered for subsequent use, and the composition content of the feed liquid is detected by high-performance liquid chromatography. The xylose content is 75.6%, and the arabinose content is 20.3%;

[0028] The hemicellulose acid hydrolyzate is pretreated by electrodialysis deionization, membrane filtration and concentration, and then enters the simulated moving bed separation as the feed liquid to eliminate interference and achieve high-efficiency separation.

[0029] (2) Start the switch of the column thermostat and the water bath box to make it rise to the set temperature of 70°C, and enter the pretreated raw...

Embodiment 2

[0033] (1) The hemicellulose hydrolyzate directly obtained by acid hydrolysis is subjected to electrodialysis, membrane filtration, concentrated pretreatment, and the measured concentration is 51%, and the high-purity water is deoxygenated and filtered for standby. The composition content of the feed liquid is detected by high performance liquid chromatography, and the content of xylose is 73.4%, and the content of arabinose is 21.8%.

[0034] (2) Start the switch of the column thermostat and the water bath box to raise it to the set temperature of 65°C, and enter the pretreated raw material liquid into the simulated moving bed chromatographic separation device for separation. The process conditions are as follows: maintain a pressure not exceeding 1Mpa Under the premise, the separation temperature is 65°C, the feed rate of the raw material liquid is 145ml / min, and the feed rate of the eluent high-purity water is 180ml / min. After 20 hours of operation, the equilibrium is reache...

Embodiment 3

[0037] (1) The hemicellulose hydrolyzate directly obtained by acid hydrolysis is subjected to electrodialysis, membrane filtration, concentrated pretreatment, and the measured concentration is 60%, and high-purity water is deoxygenated and filtered separately for standby. The composition content of the feed liquid is detected by high performance liquid chromatography, and the content of xylose is 74.6%, and the content of arabinose is 22.3%.

[0038] (2) Start the switch of the column thermostat and the water bath box to make it rise to the set temperature of 70°C, and enter the pretreated raw material liquid into the simulated moving bed chromatographic separation device for separation. Under the premise, the separation temperature is 70°C, the feed rate of the raw material liquid is 155ml / min, and the feed rate of the eluent high-purity water is 220ml / min. After 17 hours of operation, the equilibrium is reached. The conditions of the two output liquids are: one is rich in xyl...

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Abstract

The invention relates to a method for separating and purifying xylose and arabinose from hemicellulose acid hydrolysis liquid. The method comprises the following separating and purifying steps of: (1) carrying out electrodialysis and membrane filtration concentrate pretreatment on hemicellulose acid hydrolysis liquid directly obtained by adopting acid hydrolysis, deoxidizing and filtering for later use by adopting high-purity water; (2) putting the hemicellulose acid hydrolysis liquid after pretreatment into a simulated moving bed chromatography separating device and carrying out separation to obtain two discharging liquids; and (3) concentrating, cooling and crystallizing the two discharging liquids by adopting a multi-effect falling film evaporator to obtain xylose and arabinose products. In the invention, monosaccharide is extracted by adopting the simulated moving bed chromatography separation device (SSMB), a plurality of adsorption columns are serially connected into a closed loop, and inlet and outlet positions of all portions of materials can be changed by continuously switching valves, therefore, the relative motion between solid phase and liquid phase is realized, and the separation and the extraction among different components are carried out. The invention not only has the advantages of simple fixed bed adsorption operation, but also has the continuous operation capacity of a moving bed and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of simulated moving bed chromatographic separation, and relates to a method for separating and purifying xylose and arabinose from hemicellulose acid hydrolyzate. Background technique [0002] The corn deep processing industry is one of the important industries in the national economy and is closely related to people's lives. It is characterized by multiple products and full functions, and has achieved considerable development in recent years. As the main by-product of starch industry production, corn bran is a kind of cheap plant fiber raw material, which is large and concentrated. The yield of corn bran accounts for about 12% of corn (dry basis), which contains 30% to 40% of hemicellulose, which is a heterogeneous polymer composed of several different types of monosaccharides, including xylose, arbor Sugar, mannose, and galactose, among which five-carbon sugars are the main ones. After pretreatment, the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C13K13/00
Inventor 路福平孟娜李玉王春霞
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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