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Method for preparing polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode
Inactive Publication Date: 2010-08-04
WUHAN INSTITUTE OF TECHNOLOGY
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Problems solved by technology
However, the preparation process of polyaniline-enzyme modified electrodes in the prior art is generally complicated and the preparation cycle is long
Method used
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Embodiment 1
[0019] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 1 to obtain solution A, the concentration of aniline in solution A is 0.01mol / L, and the mass percent concentration of alginic acid is 0.1%;
[0020] 2) Add ionic liquid 1-butyl-3-methylimidazolium phosphorushexafluoride to solution A, and stir for 1 hour to obtain solution B, and the mass of the added ionic liquid is 2% of solution A;
[0021] 3) Add peroxidase to solution B, sonicate for 30 minutes to obtain solution C, the concentration of peroxidase in solution C is 0.01mg / mL;
[0022] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with a gold working electrode, a counter electrode and a reference electrode, the cycle potential was between -0.25-0.85V, and the number of scanning cycles was 20 circles to obtain polyaniline- Alginate-ionic liquid-enzyme composite membrane modified electrode.
Embodiment 2
[0024] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 2 to obtain solution A, the concentration of aniline in solution A is 0.03mol / L, and the mass percent concentration of alginic acid is 0.2%;
[0025] 2) Add ionic liquid 1-butyl-3-methylimidazolium phosphorushexafluoride to solution A, and stir for 2 hours to obtain solution B, and the mass of the added ionic liquid is 1% of solution A;
[0026] 3) Add glucose oxidase to solution B, sonicate for 20 minutes to obtain solution C, the concentration of glucose oxidase in solution C is 1 mg / mL;
[0027] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with a platinumworking electrode, a counter electrode and a reference electrode, the cycle potential was between -0.25-0.85V, and the number of scanning cycles was 100 cycles to obtain polyaniline- Alginate-ionic liquid-enzyme composite membrane modified electrode.
Embodiment 3
[0029] 1) dissolving aniline and alginic acid in a hydrochloric acid solution with a pH of 3 to obtain solution A, the concentration of aniline in solution A is 0.05mol / L, and the mass percent concentration of alginic acid is 0.3%;
[0030] 2) Add ionic liquid 1-butyl-3-methylimidazolium borontetrafluoride to solution A, and stir for 1 hour to obtain solution B, and the quality of the added ionic liquid is 2% of solution A;
[0031] 3) Add lactate oxidase to solution B, sonicate for 10 minutes to obtain solution C, the concentration of lactate oxidase in solution C is 0.5 mg / mL;
[0032] 4) Electrochemical cyclic voltammetry synthesis was carried out in solution C equipped with indiumtinoxide working electrode, counter electrode and reference electrode. Aniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode.
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technical field [0001] The invention relates to a preparation method of a polyaniline-alginic acid-ionic liquid-enzyme composite membrane modified electrode. Background technique [0002] The special structure and excellent physical and chemical properties of conductive polymers make them have broad and attractive application prospects in biosensors, electromagnetic shielding, metal anticorrosion and stealth technology. Among various conductive polymers, polyaniline has the characteristics of easy film formation, good film stability, and easy chemical or electrochemical synthesis. It is one of the most studied systems in the field of conductive polymers. Polyaniline-modified enzyme electrodes are a hotspot in the research of biosensors, and a variety of preparation methods have been developed. The choice of enzyme immobilization method is crucial for the preparation of sensors with fast response, high sensitivity, good stability and long service life. Alginic acid has the ...
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