Method for measuring benzo(a)pyrene in total particle phase matters in cigarette smoke gas

Abstract

The invention discloses a method for measuring benzo(a)pyrene in total particle phase matters in a cigarette smoke gas, which comprises the following steps of: preparing solution of D12-benzo(a)pyrene as internal standard solution, wherein the concentration of the solution is between 100 to 300 ng / mL; preparing six standard series solution of the benzo(a)pyrene; collecting the total particle phase matters in the cigarette smoke gas, and adding cyclohexane into the total particle phase matters to perform ultrasonic extraction; adding an extraction liquid into the internal standard solution of the D12-benzo(a)pyrene to perform vacuum concentration, and performing GC-MS analysis on a sample after the vacuum concentration; and performing internal standard method quantification. The method overcomes the defects that in the prior art, a non-target compound has large interference to a target compound, the sample pretreatment process is complex and the analysis time is long; and the method for measuring the benzo(a)pyrene in the total particle phase matters in the cigarette smoke gas is provided by the invention, which reduces the interference of the non-target compound to the target compound, simplifies the sample purification process, is more accurate in quantification, has a high recovery rate and good repeatability and can be widely applied in the field of tobaccos.

Description

technical field [0001] The invention relates to a method for measuring benzo[a]pyrene, in particular to a method for measuring benzo[a]pyrene in total particulate matter in cigarette smoke. Background technique [0002] Polycyclic Aromatic Hydrocarbons (PAHs) for short, is a relatively common carcinogenic chemical substance, which widely exists in soil, air, plants, various foods, sewage, automobile exhaust and cigarette smoke, among which benzo[ a]pyrene is a representative compound of PAHs with strong carcinogenicity. Generally speaking, other PAHs will also be detected in the sample where benzo[a]pyrene is detected. Therefore, benzo[a]pyrene is often used as PAHs representative. During the burning of cigarettes, a small amount of benzo[a]pyrene is formed due to the incomplete combustion of the high-temperature cracking of hydrocarbons in the tobacco. With the intense discussion on "smoking and health issues" worldwide, and the promulgation and implementation of the "Cig...

AI Technical Summary

Problems solved by technology

It has been reported abroad that the application of CI-source to the determination of polycyclic aromatic hydrocarbons in coal tar is very satisfactory, so we try to use gas chromatography-mass spectrometry (CI-source) to measure the content of benzo[a]pyrene in mainstream cigarette smoke. This is a new attempt, which can effectively solve the problems of cumbersome and time-consuming sample pretreatment, complex matrix and many interfering substances

[0003] It was disclosed on September 17, 2008, the publication number is CN101266230A, and the name is "a method for detecting benzo[a]pyrene in mainstream smoke of cigarettes by gel permeation-gas chromatography", which discloses a cigarette The measurement method of benzo[a]pyrene in the total particle phase of the gas, but the mass spectrometry ionization method of this prior art adopts the electron bombardment mode (EI), and the interference of the non-target compound to the target compound is large, and simultaneously from the front of the detection method In terms of processing, it needs to go through the purification process of sample solid phase extraction column purification or gel chromatography purification, the sample pretreatment process is complicated, and the analysis time is long

Images