Mutant of endotoxin neutralizing peptide and application thereof

A peptide mutant, endotoxin technology, applied in the field of peptides, to achieve the effect of improving the activity of antagonizing LPS, increasing the number of positive charges and/or hydrophobicity

Inactive Publication Date: 2010-08-11
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the neutralizing LPS activity of the LPS binding site fragment of the above-mentioned LBP or its modified po...

Method used

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  • Mutant of endotoxin neutralizing peptide and application thereof
  • Mutant of endotoxin neutralizing peptide and application thereof
  • Mutant of endotoxin neutralizing peptide and application thereof

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Embodiment Construction

[0035] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0036] 1. Experimental materials

[0037] LPS (Escherichia coli, Escherichia coli, 0111:B4), myristate-phorbol-acetate (PMA) and polymyxin B (PMB) were purchased from Sigma (USA), CE TAL chromogenic substrate Limulus reagent The box (end-point chromogenic method) was purchased from Xiamen Limulus Reagent Experimental Factory Co., Ltd.; recombinant lipopolysaccharide-binding protein (rLBP) and anti-rLBP antibody were purchased from Cell Sciences (USA), cytotoxicity detection kit (Cell countingkit-8, CCK -8) Purchased from Dojindo laboratories (JAPAN); BCA protein detection kit was purchased from Pierce Biotechnology (USA), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) detection kits were purchased from Nanjing Jiancheng Biology Institute of Engineering; human tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) enz...

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Abstract

The invention discloses a mutant of an endotoxin neutralizing peptide, which is prepared by substituting at least two of the fifth, eighth, eleventh and fifteenth amino acid residues of the endotoxin neutralizing peptide consisting of the amino acid sequence represented by SEQ ID No. 1 with the following corresponding amino acid residues: alanine which substitutes lysine, namely the fifth amino acid residue; tryptophan which substitutes phenylalanine, namely the eighth amino acid residue; lysine which substitutes glutamine, namely the eleventh amino acid residue; and lysine which substitutes aspartic acid, namely the fifteenth amino acid residue. The mutant of the endotoxin neutralizing peptide of the invention can inhibit the combination of LBP and LPS by directly neutralizing competitiveness and activity of the LPS, inhibit an LPS induced excessive inflammatory response in vitro and in vivo, has a stronger function than aparental endotoxin-neutralizing peptide from the LBP, has no visible cytotoxicity and can be applied in the preparation of medicaments for preventing and treating endotoxin associated inflammations caused by Gram-negative bacterium infection.

Description

technical field [0001] The invention relates to a polypeptide, in particular to an endotoxin neutralizing peptide mutant, and also relates to the application of the polypeptide in medicine. Background technique [0002] Endotoxin / lipopolysaccharide (LPS) is the main component of the cell wall of Gram-negative bacteria. It is composed of core polysaccharides, O-polysaccharide side chains and lipid A. pathogenic factor. Lipopolysaccharide binding protein (LBP) is a key carrier protein that mediates the binding of LPS to the target cell surface receptor CD14. The induced inflammatory cascade reaction is amplified by 100-1000 times, which can lead to systemic inflammatory response syndrome, multiple organ failure and even death in severe cases. Therefore, inhibiting the catalytic amplification effect of LBP is one of the effective strategies to prevent and treat LPS-induced sepsis. [0003] The study of the relationship between structure and function of LBP shows that the ami...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P31/04
Inventor 段光杰刘友生邓军朱江
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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