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Method for separating and purifying corn anti-hypertensive peptides

A blood pressure lowering peptide, separation and purification technology, applied in the field of separation and purification of corn blood pressure lowering peptide, can solve the problems of low purity and low activity, and achieve the effect of improving the purity and yield

Inactive Publication Date: 2010-08-11
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One object of the present invention is to provide a method for separating and purifying corn hypotensive peptides, which is used to solve the problems of low purity and low activity when separating highly active corn hypotensive peptides by the traditional method of separating corn peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. The production of corn gluten powder with zeaxanthin removed, the raw corn gluten powder is pulverized by a pulverizer and sieved, the light yellow powder after sieving is collected, and 95% ethanol is used as an extraction agent to remove zeaxanthin to obtain enzyme Solution substrate.

[0020] 2. Add alkaline protease to corn gluten powder for hydrolysis, the alkaline protease is 1% (volume ratio) of the substrate, pH 9.0, hydrolysis temperature 50°C, hydrolysis degree 8.4%, to obtain the crude hypotensive peptide.

[0021] 3. The crude hypotensive peptide is filtered through a 300-mesh plate and frame filter to remove impurities, and then filtered through a 0.45-micron membrane.

[0022] 4. Using high performance liquid chromatography to determine the molecular weight of the target product, and selecting a peptide fragment with hypotensive activity, the molecular weight of this fragment is 773. Chromatographic conditions: Chromatographic column: Agilent C18; mobi...

Embodiment 2

[0025] 1. The production of corn gluten powder with zeaxanthin removed, the raw corn gluten powder is pulverized by a pulverizer and sieved, the light yellow powder after sieving is collected, and 95% ethanol is used as an extraction agent to remove zeaxanthin to obtain enzyme Solution substrate.

[0026] 2. Add alkaline protease to corn gluten powder for hydrolysis, the alkaline protease is 1% of the substrate, pH 9.0, hydrolysis temperature 50°C, hydrolysis degree 8.4%, to obtain the crude hypotensive peptide.

[0027]3. The crude hypotensive peptide is filtered through a 300-mesh plate and frame filter to remove impurities, and then filtered through a 0.45-micron membrane.

[0028] 4. Using high performance liquid chromatography to determine the molecular weight of the target product, and selecting a peptide fragment with hypotensive activity, the molecular weight of this fragment is 773. Chromatographic conditions: Chromatographic column: Agilent C18; mobile phase: liquid...

Embodiment 3

[0031] 1. The production of corn gluten powder with zeaxanthin removed, the raw corn gluten powder is pulverized by a pulverizer and sieved, the light yellow powder after sieving is collected, and 95% ethanol is used as an extraction agent to remove zeaxanthin to obtain enzyme Solution substrate.

[0032] 2. Add alkaline protease to corn gluten powder for hydrolysis, the alkaline protease is 1% of the substrate, pH 9.0, hydrolysis temperature 50°C, hydrolysis degree 8.4%, to obtain the crude hypotensive peptide.

[0033] 3. The crude hypotensive peptide is filtered through a 300-mesh plate and frame filter to remove impurities, and then filtered through a 0.45-micron membrane.

[0034] 4. Using high performance liquid chromatography to determine the molecular weight of the target product, and selecting a peptide fragment with hypotensive activity, the molecular weight of this fragment is 773. Chromatographic conditions: Chromatographic column: Agilent C18; mobile phase: liqui...

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Abstract

The invention relates to a method for separating and purifying corn anti-hypertensive peptides, which belongs to a method for separating corn peptides from corn protein powder. The method for separating and purifying the corn anti-hypertensive peptides comprises the following steps of: hydrolyzing the corn protein powder the corn yellow pigment of which is removed by using alkali protease, filtering by adopting a plate frame filter to remove impurities, then filtering by a film, afterwards determining the molecular weight of a target product by the analysis of high-performance liquid chromatography, selecting a hydrolysate with the activity of lowering the blood pressure, and separating and purifying the hydrolysate with the activity of lowering the blood pressure by adopting a chromatographic separation system of a simulated moving bed so as to obtain the corn anti-hypertensive peptides. The separation and the purification are carried out by adopting an isocratic elution mode, wherein the feeding flow rate is 5 to 100ml / min; the flow rate of a mobile phase is 6.5 to 130ml / min, and the concentration of the mobile phase is 40 percent of ethanol; the flow rate of an outlet of a product is 4.5 to 90ml / min; and the flow rate of a circulating pump is 10 to 200ml / min, and the switching time is 6 to 120min. On the basis of achieving an industrialized level, the invention can separate the corn anti-hypertensive peptides with relatively higher purity.

Description

1. Technical field: [0001] The invention relates to a method for separating corn peptide from corn gluten powder, in particular to a method for separating and purifying corn hypotensive peptide. 2. Background technology: [0002] Corn gluten meal is the main by-product in the production of corn starch and contains about 60% protein, mainly zein (68%), glutenin (22%) and globulin (1.2%). Due to the complex composition of corn gluten meal, poor water solubility, and low content of lysine and tryptophan, its application in the food industry is limited, and because of its low price, corn gluten meal is mainly used in the feed industry in China today. This wastes precious protein resources. Enzymatic limited hydrolysis can not only improve the water solubility of zein, which is convenient for the use of limited protein resources in my country, but also obtain functional properties such as anti-oxidation, lowering blood pressure, anti-fatigue, and sobering up through further sepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
Inventor 曹龙奎刁静静
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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