Method for extracting bergapten from radix angelicae pubescentis
A technology of bergamot lactone and lodo, which is applied in the field of extraction and purification of bergamot lactone, can solve problems such as poor extraction effect, and achieve the effects of saving labor intensity, increasing content, and avoiding losses
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0024] Example 1:
[0025] Pulverize it to 60 meshes, take 10kg (bergamot lactone content 0.0046%) and add 100L 70% ethanol for reflux extraction for 3 hours, filter to obtain 104L extract, add 50g activated carbon, stir and decolorize at 40℃, pump the decolorizing solution into the pretreated Silica medium pressure column, elute with 9:1 petroleum ether / ethyl acetate mixture at a flow rate of 60ml / min, collect eluent at 500ml each, identify each component by TLC, and combine the bergamot lactone-containing segment to elute The eluate was dissolved in 10L of 70% methanol at 40°C, and allowed to stand still. The crystal was filtered out. The crystallization was repeated twice and dried in the shade to obtain 2.95 g of needle crystal product (bergamot lactone content 98.4%).
Example Embodiment
[0026] Example 2:
[0027] Pulverize it to 80 meshes, take 10kg (bergamot lactone content 0.0045%), add 150L of 74% ethanol for reflux extraction for 4 hours, filter to obtain 155L of extract, add 60g of activated carbon, stir and decolorize at 45℃, pump the decolorizing solution into the pretreated Silica medium pressure column, elute with 8:1 petroleum ether / ethyl acetate mixture at a flow rate of 60ml / min, collect the eluate in 500ml portions, identify each component by TLC, and combine the bergamot lactone-containing segments for elution The eluate was dissolved in 12L of 73% methanol at 45°C, and allowed to stand still. The crystals were filtered out. The crystallization was repeated 3 times and dried in the shade to obtain 3.08 g of needle crystal products (the content of bergamot lactone 98.1%).
Example Embodiment
[0028] Example 3:
[0029] Crush the monocle to 100 mesh, take 10kg (bergamot lactone content 0.0048%), add 180L of 75% ethanol to reflux for 5 hours, filter to obtain 185L of extract, add 80g of activated carbon and stir at 50℃ for decolorization, pump the decolorizing solution into the pretreated Silica medium pressure column, eluted with 7:1 petroleum ether / ethyl acetate mixture at a flow rate of 60ml / min, collected eluates in 500ml portions, identified each component by TLC, and merged the bergamot lactone-containing segments for elution The eluate was dissolved in 15L of 75% methanol at 50°C, and allowed to stand. The crystals were filtered out. The crystallization was repeated 4 times and dried in the shade to obtain 3.14g of needle crystal products (bergamot lactone content 98.8%).
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap