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Application of miR-145 in preparing medicament for treating inflammation

A technology of 1.mir-145, 2.mir-145, applied in the field of biomedicine

Inactive Publication Date: 2012-07-04
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that about 1% of mammalian genes encode miRNAs, which regulate 10% or more human genes. However, the target genes of most miRNAs have not yet been clarified. The discovery of miRNAs and their post-transcriptional regulation of genes are related Studying the regulation of gene expression provides important clues that can promote a better understanding of the nature of life phenomena in this field

Method used

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  • Application of miR-145 in preparing medicament for treating inflammation
  • Application of miR-145 in preparing medicament for treating inflammation
  • Application of miR-145 in preparing medicament for treating inflammation

Examples

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Embodiment 1

[0042] Example 1. Cell culture

[0043] THP-1 human macrophage cell line was cultured in RPMI 1640 (Gibco) medium containing 10% FBS in a 37°C incubator with 5% CO 2 , when the cells were in the logarithmic growth phase, 200nM 12-0-tetradecanoylphorbol-13-acetate (TPA, Cell Signaling Technology) was added to the culture medium, and differentiated into macrophages after 48 hours. HeLa and HEK 293 tumor cell lines were cultured in high-glucose DMEM (Gibco) medium containing 10% FBS in a 37°C incubator with 5% CO 2 .

[0044] 2. Isolate peripheral blood mononuclear cells, extract total RNA, and detect miR-145 by real-time PCR

[0045] After signing the informed consent, take 2ml of peripheral venous blood from 5 normal control groups, newly discovered oral impaired glucose tolerance group, and newly diagnosed type 2 diabetic patients, and separate them by Ficoll / diatrizoate density gradient centrifugation. Peripheral blood, to obtain mononuclear cells. The mirVana miRNA Isola...

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Abstract

The invention belongs to the field of biomedicine and relates to application of micromolecule RNA (Ribonucleic Acid) in preparing a medicament, in particular to action of miR-145 for treating inflammatory metabolic diseases including diabetes. Proved by a test, in the invention, under the conditions of high glucose and high fat, micro RNA participates in generation and development of inflammationlesions of metabolic diseases including diabetic vascular lesions and can be used for preparing a medicament for treating inflammation, in particular to preparing a medicament for treating the inflammatory metabolic diseases including the diabetes and a preparation for detecting the diabetes. The miR-145 can be used as a detection index of a diabetics and as a treatment target spot of inflammatory diseases including diabetic macroangiopathy.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of a small molecular RNA in the preparation of medicines, in particular to the role of miR-145 in the treatment of inflammatory and metabolic diseases such as diabetes. Background technique [0002] With changes in diet and lifestyle, metabolic diseases such as obesity, diabetes, hyperlipidemia, and hypertension have become important diseases that threaten human health. Diabetes and its chronic complications are one of the common metabolic diseases, and diabetic macrovascular complications are the main cause of death and disability in diabetic patients. A large number of previous studies have found that diabetic macrovascular lesions are mainly atherosclerotic lesions, and high sugar and high fat are the main factors leading to atherosclerotic lesions. Studies have shown that high blood sugar and high blood lipids can cause damage to endothelial cells, and monocytes adher...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61P29/00A61P3/00A61P3/10G01N33/66
Inventor 胡仁明梁文昌张伟伟杨志红
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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