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Rape-seed meal antibacterial peptide

A rapeseed cake and antimicrobial peptide technology, applied in the field of rapeseed cake antimicrobial peptides, can solve the problem of less research on plant-derived antimicrobial peptides

Inactive Publication Date: 2010-10-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Domestic research on antimicrobial peptides mainly focuses on the physical and chemical properties, mechanism of action, and application of genetic engineering of antimicrobial peptides from animals and microorganisms, and there are relatively few studies on antimicrobial peptides from plant sources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0059] 1) Medium preparation: PDA medium (potato potato medium)

[0060] 200g potatoes, 20g glucose, 20g agar, 1000mL water, natural pH;

[0061] Sterilize at 121°C for 30 minutes.

[0062] 2) Antifungal activity detection method

[0063] ① Preparation of spore suspension

[0064] Take a PDA plate covered with fungal colonies (conidia have formed), add an appropriate amount of sterile water, and gently stir with a glass rod to elute the spores. Then the eluate was filtered with 4 layers of sterile gauze to remove mycelium, and an appropriate amount of sterile water was added to prepare 3 to 5 × 10 4 spores / mL and store at 4°C. The tested strains were Green Mold citrus and Aspergillus niger.

[0065] ② punching method

[0066] Make a PDA plate, draw 100 μL of the spore suspension of the strain to be tested, operate aseptically, spread it evenly on the plate, and let it dry. Use a 6mm diameter puncher to punch holes vertically on the PDA spore plate. Add samples or steri...

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Abstract

The invention provides a new antibacterial peptide which plays a role in inhibiting molds in feed. The antibacterial peptide is derived from a rape-seed meal, has the advantages of high bacteriostatic activity, small molecular weight, high stability, safety and nontoxicity and shows better bacteriostatic effect than a single chemical mildew preventive in a feed rot test. The invention also provides a separation and purification method, a stability test method and an animal acute toxicity test method and an application method for preventing molds in the feed.

Description

1. Technical field [0001] The invention relates to rapeseed cake antimicrobial peptides which can be used to produce feed and food preservatives. 2. Background technology [0002] Adding preservatives and antifungal agents to feed is one of the effective measures to prolong the shelf life of feed. However, these feed preservatives are chemical agents, which have residual problems, produce toxic and side effects, and indirectly affect human health. Antibiotics have been added to feeds as antimicrobial growth additives for a long time. It can reduce the morbidity of animals; inhibit harmful microorganisms in the intestinal tract and improve the absorption rate of nutrients by animals, which has obvious economic benefits. However, the residues of antibiotics in animals and animal products, as well as the resistance of pathogenic bacteria, have negative impacts on human health and the environment. With the large amount and wide use of antibiotics in feed and people's higher an...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23L3/3472
Inventor 杨志荣冯甦赵建侯若彤聂远洋颜亮
Owner SICHUAN UNIV
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