Mixing hydrogen production self-flocculating bacterium particle and culture method thereof
A culture method and self-flocculation technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of low specific volume hydrogen production rate, easy washing out, etc., to improve the hydrogen production rate and wastewater treatment capacity. Improved and stable operation
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Embodiment 1
[0028] Embodiment 1, the cultivation of mixed hydrogen-producing bacteria self-flocculation particles
[0029] 1. Preparation of fermentation broth for biohydrogen production
[0030] Refer to the preparation method of biological hydrogen production fermentation liquid disclosed in the patent No. ZL00105462.7. Add 50g of soybeans and 200ml of distilled water into the brown bottle, then use N 2 O in the brown bottle 2 Drive away, stop the bottle tightly with a cork, and make the soybean undergo a hydrolysis reaction under anaerobic conditions. The temperature of the hydrolysis reaction is 5°C, and the time of the hydrolysis reaction is 2 months to obtain a biological hydrogen production culture solution;
[0031] Add 300ml of the above biohydrogen production culture solution and 700ml of distilled water into the brown bottle, and then use N 2 O in the brown bottle 2 Drive away, cork the bottle tightly, carry out fermentation culture, the temperature of fermentation culture ...
Embodiment 2
[0036] Example 2, the cultivation of mixed hydrogen-producing bacteria self-flocculation particles
[0037] 1. Preparation of fermentation broth for biohydrogen production
[0038] Refer to the preparation method of biological hydrogen production fermentation liquid disclosed in the patent No. ZL00105462.7. Add 50g corn and 200ml distilled water into the brown bottle, then use N 2 O in the brown bottle 2 Drive away, stop the bottle tightly with a cork, and make the corn undergo hydrolysis reaction under anaerobic conditions. The temperature of the hydrolysis reaction is 5°C, and the time of the hydrolysis reaction is 2 months to obtain the biological hydrogen production culture solution;
[0039] Add 300ml of the above biohydrogen production culture solution and 700ml of distilled water into the brown bottle, and then use N 2 O in the brown bottle 2 Drive away, cork the bottle tightly, carry out fermentation culture, the temperature of fermentation culture is 5 ℃, the time...
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