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Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material

A technology for bovine hemoglobin and titanium dioxide is applied in the field of preparing bovine hemoglobin and titanium-based titanium dioxide hybrid materials, which can solve the problems of inability to directly use powder performance test, increase in use cost, and incapability of continuous operation, and achieve good protein activity retention and low cost. , the effect of hydrophilic enhancement

Inactive Publication Date: 2010-10-13
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional TiO 2 Powder materials have a high specific surface area, but they cannot be operated continuously during application, and recycling and reuse will increase the cost of use
In addition, electrode materials cannot directly use powder for performance testing

Method used

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  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material
  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material
  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a. Cut the purchased titanium sheet into 1.0×2.0cm rectangular pieces, and place them in isopropanol, acetone and methanol for ultrasonic cleaning for 10 minutes. Then ultrasonically clean with deionized water for 10 minutes, at room temperature N 2 Blow dry in the atmosphere;

[0030] b. Take 0.045mol NH 4 F is dissolved in a mixed solution of 1mL deionized water and 49mL ethylene glycol to prepare a 0.09mol / L electrolyte;

[0031] c. TiO by anodic oxidation 2 Nano-array tube: connect the titanium and platinum electrodes to the anode and cathode of the DC stabilized power supply, apply a DC voltage of 40 volts for electrolysis for 6 hours, and keep the temperature of the electrolyte prepared in step b at 25°C during the whole process. After the electrolysis is completed, the sample is ultrasonically cleaned with deionized water for 30 seconds (in 3 times), and the cleaned sample is cleaned with N 2 Blow dry.

[0032] d, TiO 2 Baking of nanoarray tube: Put the sample prepared...

Embodiment 2

[0038] a. Treatment of the substrate: Cut the purchased titanium sheet into 1.0×2.0cm rectangular pieces, and place them in isopropanol, acetone and methanol for ultrasonic cleaning for 10 minutes. Then ultrasonically clean with deionized water for 10 minutes, in N 2 Blow dry in the atmosphere;

[0039] b. Preparation of electrolyte: take 0.01mol NH 4 F is dissolved in a mixed solution of 2mL deionized water and 98mL ethylene glycol to form an electrolyte;

[0040] c. TiO by anodic oxidation 2 Nano-array tube: connect the titanium and platinum electrodes to the anode and cathode of a DC power supply, apply a DC voltage of 20 volts for electrolysis for 2 hours, and maintain the temperature of the electrolyte prepared in step b at 25°C during the whole process. After the electrolysis is completed, the sample is ultrasonically cleaned with deionized water for 30 seconds (in 3 times). The cleaned sample is in N 2 Blow dry in the atmosphere;

[0041] d, TiO 2 Baking of nanoarray tube: Pu...

Embodiment 3

[0046] a. Treatment of the substrate: Cut the purchased titanium sheet into 1.0×1.0cm rectangular pieces, and place them in isopropanol, acetone and methanol for ultrasonic cleaning for 10 minutes. Then ultrasonically clean with deionized water for 10 minutes, in N 2 Blow dry in the atmosphere;

[0047] b. Preparation of electrolyte: take 0.012mol NH 4 F is dissolved in a mixed solution of 2mL deionized water and 98mL ethylene glycol to form an electrolyte;

[0048] c. TiO by anodic oxidation 2 Nanotube array: connect the titanium and platinum electrodes to the anode and cathode of the DC power supply, apply a DC voltage of 60 volts for 2 hours, and keep the temperature of the electrolyte prepared in step b at 25°C during the whole process. After the electrolysis is completed, the sample is ultrasonically cleaned with deionized water for 30 seconds (in 3 times). The cleaned sample is in N 2 Blow dry in the atmosphere;

[0049] d, TiO 2 Baking of nanotube array: Put the sample prepar...

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Abstract

The invention relates to a method for preparing a bovine hemoglobin / titanium-based titanium dioxide hybrid material and belongs to the technical field of biological-inorganic hybridization. The method comprises the following steps of: preparing a titanium-based titanium dioxide nano array tubular film; and preparing a hemoglobin and titanium-based titanium dioxide nano array tube hybrid film. A highly ordered titanium dioxide nanotube array film is prepared by an anode oxidation method and the hemoglobin is fixed to the titanium-based titanium dioxide nanotube array film by using electrostatic self-assembly technology. The method has the advantages of simple operation and good enzyme activity keeping. The prepared hybrid film can be used in the fields of bio-sensing, biological electrochemical apparatuses and the like.

Description

Technical field [0001] The invention belongs to the technical field of biological-inorganic hybridization, and particularly relates to a method for preparing a hybrid material of bovine hemoglobin and titanium-based titanium dioxide. The activity of the protein in the hybrid material is maintained well and the catalytic oxidation activity to hydrogen peroxide is good. Background technique [0002] As an important branch of supramolecular chemistry, bio-inorganic hybrid technology has always been the focus of scientific research. The construction and functionalization of bio-inorganic hybrid materials have become a research hotspot in recent and a long time in the future. Combining the characteristics of biomolecules with wide application potential, the functionalization and deviceization of biomacromolecules are realized through bio-inorganic hybrid technology, so as to realize the value of biomolecules in practical applications. Active biomolecules (such as enzymes, amino acids,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K17/14B01J31/38
Inventor 何静王艳
Owner BEIJING UNIV OF CHEM TECH
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