Different types of chitosan methacrylate and preparation method thereof

A technology of sugar methacrylate and branched methacrylic acid is applied in the field of preparation of the above-mentioned chitosan methacrylate, can solve problems such as limited application, and achieve the effects of low material cost, simple preparation steps and wide sources

Inactive Publication Date: 2010-10-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitin cannot dissolve in water, and chitosan can only dissolve in acidic solutions, which greatly limits their applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 50g of chitosan with a degree of deacetylation of 90% and a molecular weight of 50w and dissolve it in 1% acetic acid at room temperature, filter out insolubles, and prepare a 3% solution.

[0035] Add methacrylic acid to the solution at a concentration of 0.5ml / 1g chitosan; after mixing, shake at a constant temperature at 50°C and 160rpm to carry out the substitution reaction; the reaction time is 1h, and the chitosan formazan with a substitution degree of 20% is generated. Based acrylate, the product was precipitated with ethanol and freeze-dried.

[0036] Dissolve chitosan methacrylate in PBS (phosphate buffered saline) solution at pH 6, add 0.025 mol / L vitamin C and 0.062 mol / L sodium persulfate, and place at a constant temperature of 37°C for 5-15 minutes to make chitosan Sugar methacrylates polymerize to form gels.

Embodiment 2

[0038] Take 50g of chitosan with a degree of deacetylation of 90% and a molecular weight of 50w, dissolve it in 1% acetic acid at room temperature, filter off the insoluble matter, and make a 3% solution; add cellulase in the solution, and the dosage is 300U / 1g chitosan, after mixing, oscillate at 50°C and 160rpm for enzymolysis; after 3 hours of enzymolysis, the molecular weight of chitosan will be reduced to 25w; live.

[0039] Add methacrylic acid to the solution, the concentration of which is 0.8ml / 1g chitosan; after mixing, carry out the substitution reaction at 50°C and 160rpm constant temperature oscillation; the reaction time is 3h, and the chitosan formazan with a substitution degree of 40% is generated. Based acrylate, the product was precipitated with ethanol and freeze-dried.

[0040] Dissolve chitosan methacrylate in PBS (phosphate buffered saline) solution with pH 7, add 0.025 mol / L vitamin C and 0.062 mol / L sodium persulfate, and place it at a constant tempera...

Embodiment 3

[0042] Take 50g of chitosan with a degree of deacetylation of 90% and a molecular weight of 50w, dissolve it in 1% acetic acid at room temperature, filter off the insoluble matter, and make a 3% solution; add cellulase in the solution, and the dosage is 300U / 1g chitosan, after mixing, oscillate at 50°C and 160rpm for enzymolysis; after 10 hours of enzymolysis, the molecular weight of chitosan will be reduced to 10-15w; after enzymolysis, place the chitosan solution at 100°C for 10 minutes Enzyme inactivation.

[0043] Add methacrylic acid to the solution at a concentration of 0.8ml / 1g chitosan; after mixing, shake at a constant temperature at 50°C and 160rpm to carry out the substitution reaction; the reaction time is 4h, and a chitosan formazan with a substitution degree of 45% is generated. Based acrylate, the product was precipitated with ethanol and freeze-dried.

[0044] Dissolve chitosan methacrylate in PBS (phosphate buffered saline) solution at pH 8, add 0.025 mol / L ...

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Abstract

The invention relates to chitosan methacrylate with bioactivity, which is prepared by adopting the steps of: dissolving chitosan with different deacetylating degrees and different molecular weights into an acetic acid solution; adding a cellulase into the solution, uniformly mixing and then vibrating at constant temperature for enzymolysis; measuring the viscosity of the solution by using a Ubbelohde viscosity meter, calculating the molecular weight of the chitosan; after the enzymolysis, inactivating cellulase; adding methacrylic acid into the solution, uniformly mixing and then vibrating at constant temperature for substitution reaction; characterizing a grafted copolymer by using an infrared spectrum, calculating the substitution ratio of the methacrylic acid; settling chitosan methacrylate generated by the reaction by using alcohol, cooling and air-drying; and dissolving the chitosan methacrylate into different pH values of water solutions when in use, adding a crosslinking agent and placing at constant temperature to ensure that the chitosan methacrylate is polymerized into gel. The chitosan methacrylate can be used for manufacturing tissue engineering support materials, medicine slow release materials and enzyme fixing agents.

Description

technical field [0001] The invention relates to a chitosan derivative-chitosan methacrylate. [0002] The present invention also relates to a preparation method of the above-mentioned chitosan methacrylate. Background technique [0003] Chitin is a high-molecular polysaccharide polymer second only to cellulose in nature. Chitosan is a high molecular polysaccharide polymer formed after deacetylation of chitin. Because chitin and chitosan have a wide range of raw materials, and they are biocompatible, non-immunogenic and physiologically toxic, they are macromolecular substances with broad application prospects in biomedical engineering. But chitin cannot dissolve in water, and chitosan can only dissolve in acidic solution, which greatly limits their application. For this reason, chitosan can be soluble in neutral aqueous solution by substitution reaction with different chemical groups to produce different chitosan derivatives. To enable these chitosan derivatives to be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08B37/08C08K13/02C08K5/1535C08K3/30C08J3/24
Inventor 王宏田王波
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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