New application of 6-gingerol

A kind of use, the technology of gingerol, applied in the new use field of 6-gingerol, can solve the problems such as unseen 6-gingerol

Inactive Publication Date: 2010-10-20
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching the published literature of PubMed, CNKI, etc., there is no report that 6-gingerol has an effect on TRPM8 and TRPV1

Method used

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  • New application of 6-gingerol
  • New application of 6-gingerol
  • New application of 6-gingerol

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] Preparation of rat dorsal root ganglion cells (DRG): DRG cells are the main afferent neurons in the peripheral nervous system of mammals and humans, and express a variety of TRP receptor channel proteins. It plays an important role in repair, transmission of noxious stimuli, pathological development and physiological processes. In order to investigate the effect of 6-gingerol on TRPM8, rats (SD strain) suckling mice (within 5 days after birth) were selected, and the dorsal root ganglia on both sides of the spine were removed under aseptic conditions, and the remaining tissues were removed. Digest with collagenase and trypsin, centrifuge, discard the digestion solution, pipette it into a single cell suspension in conventional culture medium, count and adjust to contain 10 cells per ml 5 neurons, cultured in a culture dish pre-coated with laminin, etc., at 37°C, 5% CO 2 After culturing for 48 hours, camptothecin was used to inhibit the proliferation of other miscellaneou...

Embodiment 1

[0025] Embodiment 1: Under normal ambient temperature (37 ℃) condition, the effect of 6-gingerol on DRG cell TRPM8mRNA expression

[0026] In the purified DRG cell culture, add three different concentrations of the test compound, at 37 ° C, 5% CO 2 Cultivate for 24 hours, extract total RNA immediately after stopping the culture, set TRPV1 agonist capsaicin (control drug group) at the same time, normal culture medium without test article (normal control group), carry out fluorescent quantitative PCR amplification and TRPM8 mRNA determination. The results are shown in Table 1, 6-gingerol promotes the expression of TRPM8 mRNA, which is related to drug concentration (correlation coefficient 0.98).

[0027] Table 1: Effects of traditional Chinese medicine ingredients on TRPM8mRNA expression at 37°C (x±s) (n=8)

[0028]

Embodiment 2

[0029] Example 2: Effect of 6-gingerol on TRPM8mRNA expression in DRG cells under high temperature (39° C.) ambient temperature conditions

[0030] It has been proved by experiments that a high temperature environment (such as 39) can inhibit the expression of TRPM8. In the purified DRG cell culture, three different concentrations of the test compound were added respectively, at 37°C, 5% CO 2 Incubate for 23 hours, then at 39°C, 5% CO 2 After culturing for 1 hour, the culture was stopped, and the total RNA was extracted immediately. At the same time, the TRPV1 agonist capsaicin (control drug group) or the normal culture solution without the test substance (normal control group) were set up for fluorescent quantitative PCR amplification and TRPM8mRNA determination. . The results are shown in Table 2, 6-gingerol can down-regulate the expression of TRPM8mRNA, which is related to drug concentration (correlation coefficient -0.95).

[0031] Table 2: Effects of traditional Chines...

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Abstract

The invention discloses new application of 6-gingerol. The 6-gingerol is used for respectively regulating M8 and V1 subtype transient receptor potential ion channels (short for TRPM8 and TRPV1) of mammals including humans and can be also used for preparing medicaments for treating related diseases, such as cryalgesia allergy, the parkinsonism, painful bladder syndrome, chronic obstructive pulmonary diseases, and the like as well as tumors of skin, the prostate gland, the mammary gland, the lung, the colon, and the like, participated by the ion channels. The action intensity of the 6-gingerol is higher than that of capsaicin.

Description

technical field [0001] The invention relates to a new application of compound 6-gingerol derived from traditional Chinese medicine. Specifically, the present invention relates to the preparation of 6-gingerol in the preparation of compounds that regulate M8 and V1 subtype of Transient Receptor Potential ion channel (M8 and V1 subtype of Transient Receptor Potential ion channel) (hereinafter referred to as TRPM8 and TRPV1) and in the preparation of related pathological processes and use in medicines for diseases. Background technique [0002] Transient receptor ion channel (TRP) is a type of transmembrane structure found on the cell membrane or intracellular organelle membrane in mammalian and human cells in recent years. , opening and closing calcium ion channels, allowing calcium ions from extracellular or intracellular calcium pools to enter or exit the cytoplasm, modifying cell functions through intracellular signal transduction pathways, and participating in various pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P43/00A61P11/00A61P25/16A61P13/10A61P37/08A61P35/00A61P29/00A61P25/06A61P1/00A61P1/04A61P19/02A61P3/10A61P21/00A61P25/18
Inventor 姜廷良隋峰霍海如李兰芳郭淑英
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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