Method for extracting sodium heparin

An extraction method and heparin sodium technology are applied in the field of improvement of the enzyme-salt binding method, which can solve the problems of loss of activity and reduce the yield of heparin sodium, and achieve the effects of low production cost, high yield and stable quality.

Active Publication Date: 2010-10-20
GUANGYUAN HAITIAN IND
View PDF3 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under alkaline conditions, the N-sulfate group is quite stable, but the sulfate group on iduronic acid can be hydrolyzed, thereby inactivating it; heparin sodium can be degraded into an acidic product by the action of an oxidant, so it is purified with an oxidant , will reduce the yield of heparin sodium

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for extracting heparin sodium, comprising the following steps:

[0016] (1) Enzymatic hydrolysis of intestinal mucosal substances: Add 100 parts of intestinal mucosal substances into a reaction kettle equipped with a stirring device, a feeding device, a temperature display and an observation hole, raise the temperature to 70°C, keep warm for 15 minutes, add 180 parts of water, and add NaCl 7 part, control the temperature at 45°C, adjust the pH with lye to be 7.5, add enzyme preparation (enzyme preparation formula is: protease: papain: lipase is mixed with the mixture formed by mass parts ratio of 1: 1: 0.2, wherein protease is 2709 enzyme) 0.04 parts, stirred for 1.5 hours, added 0.27 parts of precipitant polyaluminium chloride, stirred for 10 minutes, stood for 4 hours, filtered the supernatant with a 120-mesh filter cloth, and collected the filtrate;

[0017] (2) Resin adsorption: Add 100 parts of the filtrate obtained in step (1) into a container with a stir...

Embodiment 2

[0023] The steps of the present embodiment are the same as those in Example 1, except that the formulation of the enzyme preparation and the precipitating agent are changed, and the ratio of the formulation of the enzyme preparation is changed into: protease: papain: lipase is mixed with a mixture of 4: 3: 0.3 in mass parts ratio, Wherein the protease is a mixture of AS1.398 enzyme and trypsin in a mass ratio of 1:1; the precipitating agent is changed to basic aluminum chloride.

Embodiment 3

[0025] The steps of the present embodiment are the same as in Example 1, except that the formula of the enzyme preparation and the precipitating agent is changed, and the formula of the enzyme preparation is changed into: protease: papain: lipase is mixed with the mixture formed by the mass parts ratio of 2: 2: 0.2, The protease is trypsin; the precipitating agent is changed to aluminum sulfate.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting sodium heparin, which comprises the following steps: (1) enzymic hydrolyzing intestinal mucosa in a conventional method, and adopting enzyme preparation for the hydrolysis: mixture which is formed by mixing prolease, papain and lipase at a mass ratio of 1 to 4: 1 to 3: 0.1 to 0.3, and selecting the prolease from one of 2709 enzyme, AS1.398 enzyme and pancreatin; (2) resin adsorbing in a conventional method; (3) resin eluenting in a conventional method; (4) settling out the sodium heparin in a conventional method; (5) purifying the sodium heparin in a conventional method; (6) drying the sodium heparin in a conventional method to obtain the pure sodium heparin. An improved solution is characterized in that precipitator is added before filtering and hydrolyzing the mixture in the step 1. The method has simple process, short production period, simple and convenient operation and less investment, and is applicable to the industrialized mass production. The purity of the heparin can reach 100 to 120IU/mg, the extraction efficiency can reach 100 million IU/1700 to 1800 chatterlings, the extraction efficiency can be improved by 20 to 30 percent, consumed salt can be reduced by 30 to 50 percent, water can be reduced by 50 to 70 percent, energy can be reduced by 30 to 40 percent, and the recycling rate of the crude protein can reach 60 percent.

Description

technical field [0001] The invention relates to a method for extracting heparin sodium, in particular to an improved method for enzyme-salt combination method. Background technique [0002] Heparin sodium is a sulfate ester of acidic mucopolysaccharide produced by mast cells of animal connective tissue. Because of its strong anticoagulant effect, it is the drug of choice for preventing and treating thromboembolic diseases such as deep venous thrombosis. With the development of research In-depth, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid adjustment effects, but also has various biological functions such as anti-inflammation, anti-allergy, anti-virus, and anti-cancer. Although heparin sodium has been used clinically for more than 60 years, so far there is no product that can completely replace it, so it is still the most important anticoagulant and antithrombotic biochemical drug, and currently it can only be obtained from anim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C12S3/02
Inventor 李海生
Owner GUANGYUAN HAITIAN IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap