Klebsiella pneumoniae (Mannose-sensitive hemagglutination) pilus strain
A Klebsiella, mannose technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as no medical application value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0058] Example 1. Source and identification of Klebsiella pneumoniae mannose-sensitive hemagglutination strains
[0059] The present inventor is by separating 6 strains of Klebsiella pneumoniae from clinical specimens, referring to (Muchia, the establishment of Pseudomonas aeruginosa mannose-sensitive hemagglutination pili strain, Acta Microbiology, 26 (2): 176-179 , 1986), screening and breeding were carried out to obtain a Klebsiella pneumoniae mannose-sensitive hemagglutination strain.
[0060] The main identifying features are as follows:
[0061] Colored colonies appear when lactose is fermented on differential media;
[0062] On the solid medium, gray-white sticky peptone-like colonies;
[0063] After several days of incubation in broth, the liquid becomes noticeably viscous;
[0064] Gram negative.
[0065] Electron micrographs were carried out on this strain, as shown in figure 1 Shown, showing the presence of peripilie. Mannose-sensitive hemagglutination and hem...
Embodiment 2
[0066] Embodiment 2. Bacterial strain culture and bacterial vaccine preparation
[0067] The Klebsiella pneumoniae mannose-sensitive hemagglutination pili strain of the present invention was cultivated on a bacterial plate at 37°C for 18 hours, the culture was collected, sterilized with formaldehyde solution, washed repeatedly to make a dead bacterin vaccine, diluted with sterile Bacteria physiological saline, adding 1% benzyl alcohol as a preservative to prepare the bacterin.
Embodiment 3
[0068] Embodiment 3. Animal experiment of bacterin
[0069] Use bacterial plate culture, 1% formaldehyde saline to kill bacteria, wash 5 times with sterile normal saline, spectrophotometer to detect the concentration of bacteria solution, and then check whether there are live bacteria, dilute to 1.8 to 2 billion / ml, and make bacteria Seedling.
[0070] The experimental animals used in the animal experiments are small white mice with a weight of 18-20 grams. Subcutaneous injection 3 times, each injection 0.3ml. The interval between injections was one week. Seven days after the last injection, the cross-agglutination MSHA antibody titer of the immunized mice was detected.
[0071] Table 1. After the mouse is immunized with the "bacterin" of the present invention, the cross-agglutination titer detection of the antibody in its blood and the MSHA antigen of two kinds of bacteria
[0072]
[0073] Immune protection test A total of 43 mice were immunized successively. After 1...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More