Novel klebsiella pneumoniae strain as well as isolation method and application thereof

A Klebsiella, isolation method technology, applied in the field of new Klebsiella pneumoniae strains and their isolation, can solve the problems of large harmful effects, large area of ​​pollution of phenolic industrial wastewater, and hazards of residents' food and irrigation, etc., to achieve Highly Tolerated Effects

Active Publication Date: 2018-12-21
XIAN LONGHUA ENVIRONMENTAL PROTECTION TECHCO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Phenolic industrial wastewater pollutes a wide area and has great harmful effects, which will cause serious harm to residents' diet, fish farming and crop irrigation

Method used

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  • Novel klebsiella pneumoniae strain as well as isolation method and application thereof
  • Novel klebsiella pneumoniae strain as well as isolation method and application thereof
  • Novel klebsiella pneumoniae strain as well as isolation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Isolation and identification of bacterial strain of the present invention

[0030] (1) Sampling: directly select the activated sludge from the aeration tank of the papermaking sewage treatment plant, bring the activated sludge back to the laboratory in a bucket, and inject a little activated sludge mud-water mixture into the sterilized culture medium with phenol as the only carbon source , put it into a Erlenmeyer flask to make a bacterial solution;

[0031] (2) Enrichment: Prepare 1000mL MPYE medium: 3g peptone, 3g yeast extract, 1M Mgcl 2 1.6mL, 1M CaCl 2 1.6mL, the balance is pure water; each 500mL MPYE medium is divided into 500mL blue-cap glass bottles, sealed and capped, and put into a Yamato autoclave for 30min at 121°C; take it out and put it into an ultra-clean workbench After 30 minutes of ultraviolet sterilization, the bacteria in step 1 were inoculated into a 250mL conical flask containing 100mL MPYE culture solution according to the inoculat...

Embodiment 2

[0039] Embodiment 2: new bacterial strain ZS-01 performance

[0040] (1) Phenol degradation characteristics of strains

[0041] The determination of phenol content and the preparation of the standard curve used the 4-aminoantipyrine method.

[0042] The degradation rate of phenol was calculated by the following formula:

[0043] Phenol degradation rate (%) = (A 0 -A s ) / A 0 ×100%

[0044] Among them, A 0 --The corresponding phenol content in the blank reference test without inoculation

[0045] A s --The phenol content of the sample solution to be tested that has taken over the strain

[0046] (2) Optimal degradation conditions of phenol by strain ZS-01

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Abstract

The invention discloses a novel klebsiella pneumoniae strain as well as an isolation method and application thereof. The novel strain is isolated and screened from activated sludge in an aeration tankof a papermaking sewage treatment plant and is collected in China General Microbiological Culture Collection Center, the collection name is ZS-01, the collection number is 16041, and the collection date is June 30, 2018. The strain has the characteristics and performance as follows: (1), the characteristics are: colonies formed on a phenol MedA solid culture medium are relatively short and thickbacilli, have the sizes of (0.5-0.8)*(1-2)[mu]m, are arranged separately or in pairs, and are pale yellow, uniform in texture and opaque; relatively large gray white sticky colonies are formed on an MPYE solid culture medium, are flagella-free, have capsules, belong to enterobacteriaceae and are Gram-negative short and thick bacilli; (2), the performance is: the strain has certain phenol degradingcapability and has relatively high tolerance to phenol. The strain has the advantages as follows: the strain can degrade the phenol with relatively high concentration, and provides a new bacterial source for effectively treating phenol-containing wastewater with relatively high concentration.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a new strain of Klebsiella pneumoniae and its isolation method and application. Background technique [0002] Phenol is present in various industrial wastewaters from refineries to petrochemical manufacturing. Phenol is also a major organic component in condensate streams during coal gasification and liquefaction. Phenol is also contained in industrial wastewater from pharmaceutical factories, log processing factories, paper mills, plastic factories, etc. Untreated phenolic industrial wastewater should not be discharged into open water, as it can cause odor in drinking and food processing water. The Environmental Protection Agency has established a discharge standard: the level of phenol in surface water is less than one part per billion. Phenol-containing industrial wastewater pollutes a wide area and has great harmful effects. It will cause serious harm to residents' di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/22
CPCC12N1/02C12N1/20C02F3/34C02F2101/345C12R2001/22C12N1/205
Inventor 张安龙赵呈馨王雪青王欣奇罗清房玉婷王哲毅陈朵
Owner XIAN LONGHUA ENVIRONMENTAL PROTECTION TECHCO LTD
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