Novel klebsiella pneumoniae strain as well as isolation method and application thereof

Abstract

The invention discloses a novel klebsiella pneumoniae strain as well as an isolation method and application thereof. The novel strain is isolated and screened from activated sludge in an aeration tankof a papermaking sewage treatment plant and is collected in China General Microbiological Culture Collection Center, the collection name is ZS-01, the collection number is 16041, and the collection date is June 30, 2018. The strain has the characteristics and performance as follows: (1), the characteristics are: colonies formed on a phenol MedA solid culture medium are relatively short and thickbacilli, have the sizes of (0.5-0.8)*(1-2)[mu]m, are arranged separately or in pairs, and are pale yellow, uniform in texture and opaque; relatively large gray white sticky colonies are formed on an MPYE solid culture medium, are flagella-free, have capsules, belong to enterobacteriaceae and are Gram-negative short and thick bacilli; (2), the performance is: the strain has certain phenol degradingcapability and has relatively high tolerance to phenol. The strain has the advantages as follows: the strain can degrade the phenol with relatively high concentration, and provides a new bacterial source for effectively treating phenol-containing wastewater with relatively high concentration.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a new strain of Klebsiella pneumoniae and its isolation method and application. Background technique [0002] Phenol is present in various industrial wastewaters from refineries to petrochemical manufacturing. Phenol is also a major organic component in condensate streams during coal gasification and liquefaction. Phenol is also contained in industrial wastewater from pharmaceutical factories, log processing factories, paper mills, plastic factories, etc. Untreated phenolic industrial wastewater should not be discharged into open water, as it can cause odor in drinking and food processing water. The Environmental Protection Agency has established a discharge standard: the level of phenol in surface water is less than one part per billion. Phenol-containing industrial wastewater pollutes a wide area and has great harmful effects. It will cause serious harm to residents' di...

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Problems solved by technology

Phenolic industrial wastewater pollutes a wide area and has great harmful effects, which will cause serious harm to residents' diet, fish farming and crop irrigation

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