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Method for preparing small-caliber artificial blood vessel from fish intestines
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An artificial blood vessel and small-caliber technology, which is applied in the preparation of small-caliber artificial blood vessels, and in the field of small-caliber artificial blood vessels prepared from fish intestines, can solve the problems of inability to meet the length requirements of small-caliber artificial blood vessels and difficulty in matching diameters. , to achieve the effect of excellent mechanical properties, abundant sources and low cost
Inactive Publication Date: 2012-12-12
SOUTHWEST UNIV
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The distance between the longest two branches generally does not exceed 2 cm, which means that the maximum length of the small-diameter artificial blood vessel prepared from the extracellular matrix derived from mammalian blood vessels is about 2 cm, which is obviously It cannot meet the clinical requirements for the length of small-diameter artificial blood vessels
At the same time, it is also difficult to match the pipediameter
Method used
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Embodiment 1
[0030] (1) Preparation of fish intestines:
[0031] Take an adult silver carp (Hypophthalmichthys molitrix), open the abdominal cavity, take out the fish intestine, remove the fascia and fat, remove the contents of the fish intestine, wash it, and cut it into 100 mm length.
[0033] Degreasing: Soak the fish intestines in soapy water, stir for 10 minutes every hour, and repeat the operation 12 times;
[0034] Rinsing: Take out the fish intestines, wrap them in gauze, put them under the tap, and rinse them with running water for 8 hours;
[0035] Trypsin treatment: soak the fish intestines in a trypsin solution with a concentration of 2.5g / L for 8 hours;
[0036] Compound enzyme treatment: Soak fish intestines in a solution containing 30U / ml DNase and 0.025mg / ml RNase for 4 hours;
[0037] Rinse: Take out the fish intestines, wrap them in gauze, put them under the tap, and rinse them with running water for 2 hours;
[0038]...
Embodiment 2
[0044] (1) Preparation of fish intestines:
[0045] Take an adult bighead carp (Aristichthys mobilis), open the abdominal cavity, take out the fish intestines, remove the fascia and fat, remove the contents of the fish intestines, wash and cut into 150mm lengths.
[0047] (3) Preparation of small-diameter artificial blood vessels:
[0048] Soak the extracellular matrix derived from fish intestines obtained in step (2) in PLGAchloroform solution with a concentration of 10 g / L for 1 hour, take it out, air-dry it naturally, rinse it with phosphate buffered saline (PBS) 3 times, each time 1 hours, freeze-dried, and sealed.
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Abstract
The invention applies an extra-cellular matrix from fish intestines, a brand-new good and cheap material, to research and manufacture of a small-caliber artificial blood vessel for the first time at home and abroad. The fish intestines are rich in source and good and cheap, so the prepared small-caliber artificial blood vessel has low cost. The extra-cellular matrix from the fish intestines is the same as the small-caliberblood vessel in form, so the operation is simple, convenient and feasible. The prepared small-caliber artificial blood vessel does not fracture, leak blood or cause coagulation under the blood pressure of a human body, is favorable for the conglutination of endothelial cells of the blood vessel, and can meet the requirements for small caliber and length of the blood vessel at the same time.
Description
technical field [0001] The invention relates to the technical fields of biological tissueengineering and biomaterials, in particular to a method for preparing a small-caliber artificial blood vessel, specifically a method for preparing a small-caliber artificial blood vessel using fish intestines as a raw material. Background technique [0002] Clinically, there are many vascular lesions that require vascular transplantation for patients, such as heart bypass, replantation of severed limbs, vascular defects caused by open trauma, and loss of local vascular function caused by atherosclerosis. In vascular grafting, a large number of grafts are required. The best source of these grafts is blood vessels from other parts of the patient's body, however, this will lead to varying degrees of damage to the body at the site where the grafts are taken. For this reason, people turn their attention to allogeneic blood vessels, which in addition to facing the problem of limited donors I...
Claims
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Application Information
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