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Preparation of novel high-efficiency biocatalyst based on porous microcapsule technology

A biocatalyst and a new type of technology, applied in the direction of microorganism-based methods, microorganisms, microcapsule preparations, etc., can solve problems such as collapse, loose structure, and instability of calcium alginate, and achieve high mechanical strength, high cell loading capacity, and pass through good permeability

Inactive Publication Date: 2010-12-01
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its disadvantage is that calcium alginate is unstable in phosphate buffer and solution, and decalcification makes the structure loose and collapse.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Use purified water to prepare a 15% PVA solution and a 5% sodium alginate solution, and sterilize it in an autoclave at 121°C for 20 minutes; take 70ml of a 15% PVA solution and mix it with 5% alginate 30ml of sodium solution is fully mixed and prepared into capsule shell material and placed in the capsule shell material storage container of the outer layer (coating layer) of the coaxial double-layer tube; in addition, 20 grams of brewer's yeast cells are put into 0.2% CTAB solution Carry out permeabilization treatment for 20 minutes, wash the permeabilized brewer's yeast cells repeatedly with deionized water 3 times, then centrifuge at 3000rmp for 5 minutes, put the centrifuged brewer's yeast cell liquid into the coaxial double-layer tube Layer (inner core fluid), that is, in the inner core fluid storage container; under a certain pressure, the material in the coaxial double-layer tube is squeezed out at the same time, and sterilized 4% CaCl is dripped 2 Solidification...

example 2

[0015] Prepare a 10% PVA solution and a 10% sodium alginate solution with purified water, and sterilize in an autoclave at 121°C for 20 minutes; take 85ml of 10% PVA solution, mix with 10% alginate 15ml of sodium solution is fully mixed and prepared into capsule shell material and placed in the capsule shell material storage container of the outer layer (coating layer) of the coaxial double-layer tube; in addition, 20 grams of baker's yeast cells are put into 0.5% CTAB solution Perform permeabilization treatment for 10 minutes, wash the permeabilized brewer's yeast cells repeatedly with deionized water three times, then centrifuge at 3000rmp for 5 minutes, and put the centrifuged brewer's yeast cell liquid into the coaxial double-layer tube layer (inner core fluid), that is, in the inner core fluid storage container; under a certain pressure, the material in the coaxial double-layer tube is squeezed out at the same time, and sterilized 2% CaCl is dripped 2 Solidification and m...

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Abstract

The invention relates to a new method for preparing a novel high-efficiency porous microcapsule biocatalyst by combining a coaxial double-layer technology and an in-situ erosion punching technology. A microcapsule with a diameter of 3-4mm is firstly prepared, wherein the inner core of the microcapsule comprises permeability beer or bread yeast cells, and an outer-layer shell of the microcapsule is prepared by polyvinyl alcohol / calcium alginate mixed gel; and finally, the mechanical strength of an outer layer of the microcapsule is increased by further solidifying through freezing and thawing repeatedly. Because cellular fluid is in the microcapsule, the microcapsule biocatalyst which is similar to a micro-bioreactor is formed; in addition, a phosphate solution or other calcium-ion complexing agents are used for carrying out chemical erosion to dissolve partial calcium alginate gel by the in-situ erosion punching technology in order to achieve an in-situ erosion punching purpose, thereby a microchannel is formed to promote the transmission of micromolecular materials and products in a gel coating layer, and moreover, the catalytic activity of cells in the porous microcapsule core is increased by the permeability treatment of the beer or bread yeast cells. Due to the adoption of the method, the novel high-efficiency porous microcapsule biocatalyst has high cell capacity, strong catalytic effect, low diffusional limitation and high mechanical strength and is used to prepare important chiral drugs, such as chiral alcohol, and the like and a medical and chemical intermediate.

Description

technical field [0001] The invention relates to a new technology of "constructing high-efficiency biocatalyst based on novel porous microcapsule technology". Background technique [0002] Microcapsules in the biological field are spherical microcapsules formed by encapsulating biological macromolecules such as enzymes, coenzymes, proteins, or microorganisms, animal and plant cells in a hydrophilic semi-permeable membrane, so that biological macromolecules or cells are blocked In the membrane of the microcapsule, oxygen, nutrients and other small molecular substances can freely enter and exit the capsule membrane for material transfer, so as to achieve the purpose of catalysis, cultivation or immune isolation. Due to the characteristics of a semipermeable microcapsule membrane and a liquid environment in the membrane, biological microcapsules have the function of protecting enzyme molecules or cells in the membrane and controlling the transfer of substances through the capsul...

Claims

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Application Information

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IPC IPC(8): C12N11/04B01J13/02C12R1/645C12R1/865
Inventor 于明安龚耿浩侯毅赵泉王施韦黎刚
Owner CHONGQING MEDICAL UNIVERSITY
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