Method of building new high-efficiency biocatalyst based on tissue engineering method

Abstract

The invention relates to a new method of building a new high-efficiency biocatalyst through the combination of tissue engineering and chemical technology. The method comprises the following steps: using microorganisms as seed cells to expand culture, inoculating on scaffold material, performing tissue culture to prepare a tissue engineering cell sheet with the structure of cell-scaffold three-dimensional space complex, wherein the cell capacity is up to over 80% and enough channels are left in the scaffold for substrate or product to pass in and out; coating the cell sheet with polyvinyl alcohol / sodium alginate, forming in calcium chloride solution, freezing and thawing to form a gelatin coated tablet, wherein partial calcium alginate in the coating layer is dissolved in phosphates to form microchannels used for micromolecular transfer; and then performing permeabilization to increase the endoenzyme in the gelatin coated tablet to build the permeabilized tissue engineering cell gelatin coated tablet which is a new high-efficiency biocatalyst and has high cell capacity, strong catalytic effect, low diffusional limitation and good mechanical strength. The new high-efficiency biocatalyst can be used to replace the traditional immobilized cell and prepare important chiral drugs and pharmaceutical and chemical intermediates such as chiral alcohols through biocatalysis.

Description

technical field [0001] The invention relates to a new technology of "constructing a novel high-efficiency biocatalyst based on a tissue engineering method". Background technique [0002] The concept of tissue engineering was first proposed by Biscgelie as early as 1933, but it was not until 60 years later in 1993 that Langer and Vacanti published a paper in the journal "Science" to give a clear definition: tissue engineering is a multidisciplinary research field that applies life Science and engineering principles to develop biological substitutes that restore, maintain or improve tissue function. Since then, tissue engineering has successfully expanded and inspired scientists and clinicians. It is a new technology proposed to solve the shortage of mammalian tissue resources, high cost, and possible complications of transplantation. In the past 15 years, tissue engineering has developed into a new discipline, and its research covers various tissues and organs of the human ...

AI Technical Summary

Problems solved by technology

However, its disadvantage is that calcium alginate is unstable in phosphate buffer and solution, and decalcification makes the structure loose and collapse.