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Method for extracting rhoifolin and prepared medicament application thereof

A technology of Rhodorin and its extraction method, which is applied in the field of Rhodorin extraction, and can solve the problems of high price and the like

Active Publication Date: 2013-07-17
JIANGXI SHANXIANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Western medicine "Oseltamivir Phosphate (Tamiflu, (3R, 4R, 5S)-4-Acetamide-5-Amino-3(1-Propoxyethyl)-1-Cyclohexene-1 Carboxylic Acid Ethyl Phosphate "Salt)" is a neuraminidase inhibitor, which has a specific inhibitory effect on the entry of influenza virus into cells, but it is not only expensive, but also some patients experience vomiting, nausea, insomnia, headache, abdominal pain, diarrhea, dizziness, fatigue, Side effects such as nasal congestion, sore throat, and coughing

Method used

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  • Method for extracting rhoifolin and prepared medicament application thereof
  • Method for extracting rhoifolin and prepared medicament application thereof
  • Method for extracting rhoifolin and prepared medicament application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Take 1000g of the round leaves of Shanxiang, add 12 times the amount of 70% ethanol for reflux extraction for 2 hours, filter, add 10 times the amount of 70% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 10% ethanol, 45% ethanol, and 1% sodium hydroxide solution respectively, and the eluted part of 45% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the Rhodorin fractions were combined and crystallized to obtain the pure Rhodoglucoside (1.5g, purity: 98.5%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was determined.

Embodiment 2

[0032]Take 1000g of the round leaves of Shanxiang, add 10 times the amount of 60% ethanol for reflux extraction for 2 hours, filter, add 8 times the amount of 60% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 10% ethanol, 45% ethanol, and 0.8% sodium hydroxide solution respectively, and the eluted part of 45% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the rhodium glucoside fractions were combined and crystallized to obtain pure rhodium rhoside (1.6g, purity: 98.7%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was determined.

Embodiment 3

[0034] Take 1000g of the round leaves of Shanxiang, add 15 times the amount of 60% ethanol for reflux extraction for 2 hours, filter, add 12 times the amount of 60% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 8% ethanol, 40% ethanol, and 1.2% sodium hydroxide solution respectively, and the eluted part of 40% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the rhodium glucoside fractions were combined and crystallized to obtain the pure rhodium rhoside (1.7g, purity: 98.9%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was determined.

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Abstract

The invention relates to a rhoifolin and a prepared medicament application thereof. The method for extracting the rhoifolin comprises the following steps of: taking turpinia formosana leaves or exocarpium citrl grandis, adding 5-15 times of 30-90 percent ethanol for carrying out refluxing extraction for 1-3 times and filtering, wherein each refluxing extraction is carried out for 1-3 hours; combining filter liquid and recycling ethanol; eluting an aqueous solution by a macroporous resin column, collecting an eluted part, depressurizing for concentrating and drying to obtain the mixed total glycoside of the rhoifolin; and separating, combining rhoifolin fractions and crystallizing to obtain pure rhoifolin. The rhoifolin can be used as a neuraminidase inhibitor for preventing and treating influenza and made into a pharmaceutically acceptable formulation.

Description

technical field [0001] The invention relates to a method for extracting rhodol and its use in the preparation of medicines. Background technique [0002] At present, influenza virus is becoming more and more serious, and influenza virus is closely related to respiratory diseases and systemic diseases caused by it. my country is one of the countries with high incidence of influenza. Not only has a large population, but also living habits are conducive to the transmission of influenza virus. The average number of episodes ranged from 0.3 to 0.7, and 2 to 4 in key groups. Influenza poses a serious threat to human beings, especially the emerging strains of influenza virus, which not only cause secondary infections of other microorganisms, but also directly lead to organ damage and allergic reactions leading to death. But there is no effective method and treatment means for treating influenza virus and its disease (influenza) at present, such as treating the inflammatory response...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P31/16A61P13/12A61P1/00A61P11/00
Inventor 杨小玲王燕平谢宁叶劲英李志勇吕武清刘地发程帆蔡永红
Owner JIANGXI SHANXIANG PHARMA
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