Method for generating large-scale sympodial bamboos through Agrobacterium mediation
An Agrobacterium-mediated method and Agrobacterium-mediated technology are applied in the field of producing large-scale clump bamboos using the Agrobacterium-mediated method, which can solve the problems of restricting gene expression and regulatory applications, and achieve the goal of increasing the probability, shortening the cycle, and broad application prospects Effect
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[0032] Below in conjunction with embodiment the present invention is further described.
[0033] Agrobacterium-mediated method to produce the method for large cluster bamboo, comprising the steps:
[0034] a. callus induction: take mature embryos of bamboo seeds and place them on the callus induction medium MS2 to culture them for 2-3 weeks to obtain callus tissue, and after 3-4 weeks, obtain bamboo embryogenic callus tissue;
[0035] b. Pre-cultivation: transfer the bamboo embryogenic callus in the late stage of induction to the fresh callus induction medium MS2 for pre-cultivation for 1 week;
[0036] c. Activation and resuspension of Agrobacterium containing the target gene: Pick the Agrobacterium containing the target plasmid, streak it on the Agrobacterium medium YEB containing 50mg / L rifampicin and 100mg / L kanamycin, and store it at 28°C Cultivate in the incubator for two days; pick a single colony and continue to culture it on another fresh Agrobacterium medium YEB con...
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