Chronic digestible starch and preparation method thereof

A technology for digesting starch and chronicity, which is applied in the field of chronic digestion starch and its preparation, can solve the problems of harmful chemicals and complex preparation methods, and achieve the effect of wide application and slow down oxidation

Inactive Publication Date: 2010-12-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there are defects in the above-mentioned prior art that the preparation method is c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Determination of slow digestible starch content:

[0034] Refer to the in vitro simulated enzymatic hydrolysis method proposed by Englyst, and improve it. Accurately weigh 6000 mg of slow-digestible starch sample into a 50 mL centrifuge tube with a rotating cap, add 10 mL of deionized water, vortex and mix well, and keep in a boiling water bath for 30 min. After cooling to room temperature, add 10 mL of 0.1 mol / L sodium acetate buffer solution with pH 5.2 and 5 glass beads, mix well and shake in a constant temperature water bath at 37°C. Add 5 mL of mixed enzyme containing porcine trypsin and amyloglucosidase, place in a constant temperature water bath at 37°C and shake and time accurately. After 20-120 min of hydrolysis, 1.00 mL of hydrolyzed solution was taken out and the glucose content was determined by the 3,5-dinitrosalicylic acid (DNS) method. The calculation formulas of rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) a...

Embodiment 2

[0043](1) High-chain corn starch (Hylon V, wherein the amylose mass percentage content is 50%) and acetate buffer solution of pH 4.0 are formulated into starch milk with a mass fraction of 5%, kept stirring, and placed in a boiling water bath Heat at medium temperature for 15 minutes to gelatinize the starch, and cool to 50°C after gelatinization;

[0044] (2) Add 5 ASPU / g of pullulanase to the cooled starch paste, mix well, and carry out heat preservation enzymatic detachment for 2 hours;

[0045] (3) After debranching, the temperature is raised to 60° C., lauric acid with 5% starch dry basis weight is added, and reacted for 30 minutes; finally, it is rapidly cooled to room temperature, centrifuged at a speed of 4000 rpm for 20 minutes, washed with an ethanol solution with a concentration of 50% by volume, and pumped. Filter and dry at a low temperature of 40°C to obtain a slowly digested starch product.

[0046] The SDS content of the obtained slowly digested starch product...

Embodiment 3

[0048] (1) High-chain corn starch (Hylon V, wherein the mass percentage of amylose is 50%) and acetate buffer solution of pH 4.5 is formulated into starch milk with a mass fraction of 10%, kept stirring, and placed in a boiling water bath Heat at medium temperature for 30 minutes to gelatinize the starch, and cool to 60°C after gelatinization;

[0049] (2) Add 10 ASPU / g of pullulanase to the cooled starch paste, mix well, and carry out heat preservation enzymatic debranching for 12 hours;

[0050] (3) After debranching, heat up to 70°C, add palmitic acid with 10% starch dry basis mass, and react for 60min; finally cool down to room temperature rapidly, centrifuge at a speed of 4000rpm for 20min, wash with ethanol solution with a concentration of 50% by volume, and pump Filter and dry at a low temperature of 40°C to obtain a slowly digested starch product.

[0051] The SDS content of the obtained slowly digested starch product was determined to be 18.6%.

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Abstract

The invention discloses chronic digestible starch and a preparation method thereof. The method comprises the following steps of: (1) preparing starch emulsion from starch and buffer solution, keeping stirring, heating the emulsion in a boiling water bath into paste, and cooling the past; (2) adding pullulanase into the cooled starch paste, uniformly mixing and performing heat-preserving enzymolysis debraching treatment; and (3) heating the product obtained by the step (2), adding fatty acid, and quickly cooling to room temperature after reaction, centrifuging, washing a precipitate by using ethanol, filtering, and drying at low temperature to obtain the chronic digestible starch. The chronic digestible starch product has the characteristics of slow digestion, reinforced nutrition and the like, and can be widely applied in the fields of functional foods, heal-care products and the like.

Description

technical field [0001] The invention relates to the field of starch modification and its deep processing, in particular to a slowly digested starch and a preparation method thereof. Background technique [0002] With the emergence of various diseases of modern civilization, people have a deeper understanding of carbohydrates as the main energy source of the human body. British physiologist Englyst divides starch into rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) according to the digestion of starch in the human body. Fast digestible starch refers to those starches that can be quickly digested in the mouth and small intestine (<20min); slow digestible starch refers to a kind of starch that can be completely digested in the small intestine but the digestion rate is relatively slow (20-120min); resistant starch Upper refers to the starch (>120min) that cannot be absorbed by the human small intestine and is finally fermented and...

Claims

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Application Information

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IPC IPC(8): C08B33/02C08B30/20C12S3/12A23L1/308A23L33/21
Inventor 黄强罗发兴张斌
Owner SOUTH CHINA UNIV OF TECH
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