Preparation method of recombinant tumor specificity antiapoptotic factors with activity and application of products thereof

Abstract

The invention provides a preparation method of recombinant tumor specificity antiapoptotic factors with activity and an application of products thereof, and the key points are as follows: (1) gene segments of Apoptin are directly obtained in an artificial synthesis method, and a prokaryotic expression vector pGEX-A comprising GST-Apoptin gene is constructed; (2) GST-Apoptin fusion protein is fermented, efficiently expressed, dissolved, renatured and purified; and (3) the GST-Apoptin fusion protein is chemically modified, thus obtaining the recombinant tumor specificity antiapoptotic factors with the activity, which can induce apoptosis of tumor cells and are applied to anti-tumor therapy. The invention has the beneficial effects of having efficient expression, good stability, high purity and high activity in Escherichia coli and being suitable for goingtoscale and industrialization.

Description

technical field [0001] The invention relates to a preparation method and application of biopharmaceuticals, especially a preparation method of an active recombinant tumor-specific apoptosis factor and the application of the product obtained by the method in anti-tumor treatment. Background technique [0002] Cancer treatment is a worldwide problem. Cancer biological treatment is an important supplement to surgery, radiotherapy, chemotherapy and other methods. Finding more effective biological agents has become one of the key research fields in the 21st century. At present, the biological agents for tumor treatment that have been marketed at home and abroad mainly include interleukin-2 (IL-2), interferon (IFN) and monocyte colony-stimulating factor (GSM). There are few varieties, and they are all immune enhancers or modulators, so the research and development of new biological agents with therapeutic effects has important social and economic significance. [0003] Chicken an...

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Problems solved by technology

[0007] (3) Although there are a large number of existing antineoplastic drugs, there are some with better curative effect, such as paclitaxel, arsenic preparations, etc., but most of the drugs have severe side effects, and drug resistance often occurs after repeated medication, which leads to tumor recurrence. It is very necessary to develop anti-tumor drugs with less toxic side effects and significant curative effect

Due to its high technical and equipment requirements, this method is only suitable for laboratory research and cannot be applied on a large scale, so it cannot be industrialized

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