Production method of compound amino acid feed additive rich in gamma-aminobutyric acid (GABA)

Abstract

The invention discloses a production method of a compound amino acid feed additive rich in gamma-aminobutyric acid(GABA), which comprises the following steps: concentrating mixed amino acid mother liquor, which serves as a raw material and is obtained from the hydroxylation production of L-cystine or extraction of other monomer amino acids, under reduced pressure, filtering concentrate, diluting filtrate with water, decolorizing with active carbon, adjusting pH value to 5 to 7 with acid or alkali, adding glutamate decarboxylase in an amount which is 5 to 10 percent of the volume of the mother liquor obtained after pH adjustment and a phosphopyridoxal added coenzyme factor in an amount which is 0.05 to 0.1 weight percent, fermenting at constant temperature for 15 to 20 hours, concentrating under reduced pressure or with an RO reverse osmosis membrane, and spray drying to obtain the compound amino acid feed additive rich in GABA. The method has the advantages that: the problem of low GABA yield and high production cost is solved; the process is simple; the cost is low; waste mother liquor is utilized comprehensively; and resources are saved.

Description

technical field [0001] The invention belongs to the field of production methods of feed additives, in particular to a production method of feed additives rich in gamma-aminobutyric acid. Background technique [0002] At present, L-cystine is mostly produced by protein hydrolysis in China. Protein hydrolysis can produce 18 kinds of amino acids such as L-cystine, L-glutamic acid, and L-arginine, but it has industrial production value and has been able to produce Only L-cystine, L-tyrosine, L-leucine, L-arginine, L-histidine, L-histidine, L-lysine and other six amino acids are isolated and extracted. The total amino acid extraction rate is less than 20%, and the remaining more than 80% of the amino acid exists in the mixed mother liquor, or is discharged or dried to make low-grade compound amino acid powder for sale as a feed additive. my country's current annual output of L-cystine is about 6,000 tons, and each ton of cystine by-product compound amino acid mother liquor is 35...

AI Technical Summary

Problems solved by technology

However, it is not easy to produce GABA by the above methods. It is necessary to add L-glutamic acid and its salts with high price, and it is difficult to increase its concentration and purification technology. Therefore, the produced GABA is generally more expensive. It is used in general animal feed It is difficult to directly add GABA. In order to add GABA to feed for livestock, it is necessary to provide an easy-to-obtain and relatively cheap production method of GABA-rich feed additives.