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Technology for highly-efficiently enriching pathogenic bacteria in water environments

A technology for pathogenic bacteria and water environment, applied in the fields of filtration and separation, separation of microorganisms, chemical instruments and methods, etc., can solve the problems of low content of pathogenic microorganisms and low detection sensitivity, and achieves simple operation, large amount of water samples, high efficiency high effect

Inactive Publication Date: 2010-12-29
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, the content of pathogenic microorganisms in the water environment is very small, which is far lower than the detection sensitivity of existing monitoring methods

Method used

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  • Technology for highly-efficiently enriching pathogenic bacteria in water environments
  • Technology for highly-efficiently enriching pathogenic bacteria in water environments
  • Technology for highly-efficiently enriching pathogenic bacteria in water environments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] To make a positively charged filter material, the main steps are as follows:

[0043] ① Preparation of Magnesium Aluminum Hydroxide Colloid

[0044] a. Weigh 192 grams of AlCl respectively 3 ·6H 2 O and 488 g MgCl 2 ·6H 2 O was dissolved in 8L deionized water, and under stirring at 300 rpm, slowly and uniformly added about 2.4L of 3mol / L NaOH solution until no precipitation occurred. Adjust the pH to about 8.0 with hydrochloric acid, continue to stir for 2 hours, and then carry out static aging.

[0045] b. During the static aging process, pour out the supernatant the next day, make up the volume with deionized water, and stir evenly. A total of 3 water changes were performed, lasting about 5 days.

[0046] ② Preparation of filter material

[0047] a. Centrifuge the aged magnesium aluminum hydroxide colloid with a centrifugation parameter of 6000rpm×10min. Pour off the supernatant and keep the magnesium aluminum hydroxide precipitate.

[0048] b. Put the magnes...

Embodiment 2

[0053] The main implementation steps for making the filter device and building the filter system are as follows:

[0054] ①Filter production

[0055] a. Take a plexiglass tube with a diameter of 6cm and a length of 20cm, and insert one end of it into a hard plastic tube with a diameter of 0.5cm and a length of about 2cm as the outlet pipe. About 1cm away from the water outlet, fix a 200-mesh stainless steel mesh with the same inner diameter as the plexiglass tube to carry the filter material and filter the water. The other end of the plexiglass tube is machined with a section of thread, and a plastic nut with the same diameter is provided. A small hole is opened in the middle of the nut and a hard plastic water inlet pipe with a diameter of 0.5 cm and a length of 2 cm is installed.

[0056] b. Open the nut and load the magnesium aluminum hydroxide filter material with a height of about 15cm, and then re-tighten the nut.

[0057] ②Establishment of filtration system

[0058] P...

Embodiment 3

[0060] To prepare the eluent, the main steps are as follows:

[0061] a.3.723gNa 2 HPO 4 and 9.238 g NaH 2 PO 4 Completely dissolve in 1L deionized water to make a buffer solution with a pH value of about 6.5.

[0062] b. Add 5mL of analytically pure tween-80 to 1L of the above buffer solution, and stir well.

[0063] c. Dispense the prepared eluent into test tubes with 10 mL each, and autoclave at 121°C for 20 minutes.

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Abstract

The invention discloses technology and methods for highly-efficiently enriching and recovering pathogenic bacteria in water environments. The technology comprises the following main contents of: (1) the method for preparing a novel positive charge-carrying filter material; (2) the method for manufacturing a filter; (3) the assembly of a filtering concentration system; (4) the method for preparingan eluent; and (5) the method for concentrating and recovering the pathogenic bacteria. Through the technology and the methods of the invention, the pathogenic bacteria can be highly-efficiently enriched and recovered from various water environments such as tap water, water in clean water sources, heavily-polluted water bodies and sewage, the concentration and recovery efficiency is stable, the recovery rate of cleaner water bodies can reach over 90 percent, and the recovery rate of the heavily-polluted water bodies can reach over 80 percent. The filter material in the invention can be used repeatedly after being simply treated, and other facilities can also be used repeatedly, so the technology and the methods of the invention have the advantages of simplicity, economy and practicality, and are particularly suitable for use in laboratories and at site.

Description

technical field [0001] The invention relates to a method for efficiently enriching pathogenic bacteria from water environment. Background technique [0002] Water is a substance that human beings depend on for survival. It is not only used for drinking and domestic use, but also a discharge site for human and animal excrement, production and domestic sewage, etc. Therefore, there are various microorganisms in water. The greatest threat to human health in water is pathogenic bacteria: in history, there have been many worldwide cholera pandemics; 139 ) pollution has led to a new cholera epidemic; in addition, due to natural disasters, wars, sanctions, economic backwardness caused by the deterioration of sanitation, the decline in drinking water quality caused other bacterial diseases (such as typhoid, bacillary dysentery, diarrhea, etc.) Outbreaks are also very common. According to the WHO annual report, children under the age of 5 in Asia (excluding China), Africa, and Lati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02B01D39/06
Inventor 邱志刚李君文王景峰王新为金敏谌志强陈照立
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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