Multi-phosphopeptide-enriched casein hydrolyzate and preparation method thereof

A casein hydrolyzate and proteolytic enzyme technology, applied in peptide sources, animal/human peptides, fermentation, etc., can solve problems such as sequence structure analysis, achieve high activity, good research samples, and good application prospects

Inactive Publication Date: 2011-01-19
TIANJIN UNIV
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, researchers have reported the separation and purification of casein phosphopeptides, but s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-phosphopeptide-enriched casein hydrolyzate and preparation method thereof
  • Multi-phosphopeptide-enriched casein hydrolyzate and preparation method thereof
  • Multi-phosphopeptide-enriched casein hydrolyzate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] Prepare 1L of 20g / L cow casein (Sigma) solution, adjust the pH to 8.0, and place it in a reactor with continuous stirring and a constant temperature of 37°C. Add 0.2 g of pancreatin (Sigma) according to the mass ratio of enzyme to substrate of 1:100, and start timing, while adding NaOH dropwise to maintain the pH of the system at 8.0. The reaction was stopped after 10 minutes of reaction, and the enzyme was inactivated in a boiling water bath for 10 minutes, at which time the degree of hydrolysis was 6%. The enzymolysis solution is filtered with a 5kDa hollow fiber membrane to obtain a filtrate (product), and then a 1kDa hollow fiber membrane is used for filtration to obtain a retentate with a molecular weight of 1k-5kDa. Add CaCl to the retentate 2 Make the concentration 1.0%, let it stand for 60 min, add ethanol to make the ethanol concentration 60%, and form a precipitate, which is centrifuged (10000 rpm) and then the precipitate is taken.

[0025] Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a multi-phosphopeptide-enriched casein hydrolyzate and a preparation method thereof. The method comprises the following steps of: with the casein as a raw material, suitably hydrolyzing by adopting a proteolytic enzyme, controlling a hydrolysis degree within 10 percent, heating with a boiling water bath for killing enzyme and separating out various polypeptide products from a reaction system by adopting an ultrafiltration membrane of 5 k Da; then, intercepting the product with a hyperfiltration membrane of 1 k Da and selectively precipitating phosphoeptide from the obtained intercepting liquid by adopting calcium-ethanol; dissolving the phosphoeptide with deionized water or a volatile salt solution, sampling on a strong cation exchange column and separating to obtain a plurality of phosphorylated peptides; acquiring a product by adopting spraying drying or freezing drying; and finally, carrying out the sequence analysis on the obtained phosphoeptide by adopting LC/MS (Coupled HPLC-Mass Spectrometry). The invention can selectively acquire the phosphoeptide with higher activity, has favorable application prospects in the field of food biotechnology and provides a favorable research sample for researching phosphoproteomics.

Description

technical field [0001] The invention relates to a casein hydrolyzate rich in polyphosphorylated polypeptides, and a preparation method thereof. Most of these phosphopeptides carry SerP-SerP-SerP-Glu-Glu sequence fragments, and belong to the field of food biotechnology. Background technique [0002] Casein accounts for about 80% of the total protein in mammalian milk and is composed of α S1 - casein, alpha S2 -A micellar macromolecule formed by the aggregation of four phosphorylated proteins such as casein, β-casein, and κ-casein. When newborns are fed breast milk, casein micelles act as calcium phosphate colloidal transporters, providing essential mineral elements for the child's bone formation and tooth growth. During the digestion of breast milk in the body, casein will be hydrolyzed into various polypeptides, but its metal-binding function will be retained or even strengthened by the hydrolyzate-casein phosphopeptides (CPPs). At present, a large number of experimental ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/47C07K1/36C07K1/34C07K1/30C07K1/18C12P21/06
Inventor 苏荣欣齐崴何志敏
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap