Kit for detecting free thyroxin by using magnetic particles as solid-phase carriers and preparation method thereof

Abstract

The invention discloses a kit for detecting free thyroxin by using magnetic particles as solid-phase carriers. The kit comprises magnetic particle suspension coupled with triiodothyronine-gelatin compound, free thyroxin series calibrator, horseradish peroxidase labeled antithyroidin antibody solution, luminous substrate liquid A and liquid B and concentration washing liquid. The invention also discloses a preparation method for the kit. The kit has the advantages that: because a magnetic particle chemical luminescent method is combined with a magnetic particle solid-phase coupling technique, the magnetic particles are suspended in the liquid in the absence of magnetic field, and the antigen antibody reaction is similar to homogeneous reaction; and the magnetic particles are convenient to separate under the action of an external magnetic field and quick to wash, and can improve the detection precision and stability when used as immunological carriers. The kit has the advantages of simple composition, convenient operation, stable and reliable result; and compared with the commercial elisa kits and plate-type chemical luminescent kits, the kit has the advantage that the reaction timeis only 1 / 8 to 1 / 4 of the time of the kits.

Description

technical field [0001] The invention relates to clinical immunoassay technology, in particular to a kit for detecting free thyroxine using magnetic microparticles as a solid phase carrier, and also relates to a preparation method of the kit. Background technique [0002] Thyroxine (T4) is a thyroid hormone synthesized and secreted by thyroid follicular epithelial cells. Its structure consists of a tyrosine residue and a phenolic ring. an iodine atom. The average daily T4 secretion of normal people is 90±9μg, which is about 1% of the storage capacity in the thyroid gland. After entering the blood circulation, about 99.96% of T4 binds to binding proteins, of which about 60% binds to TBG, 30% binds to TBPA, and the rest binds to albumin. There is a reversible equilibrium between bound T4 and free T4. Thyroxine in the bound state cannot exert its physiological functions, but only free T4 can enter the target cells to bind with T4 receptors and exert its physiological function...

AI Technical Summary

Problems solved by technology

[0005] Thyroxine is a small molecular substance, and immunoassay is determined by competition method. When free thyroxine is measured, the enzyme-labeled antigen will bind to the protein in the serum like the analyte, so that the markers involved in the immune response will be reduced, resulting in the measurement result mistake