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Tissue culture quick propagation method for Opisthopappus Shih

A tissue culture, Taihang chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as operation methods and techniques that do not provide implementable guidance, and achieve strong implementability and repeatability. , the effect of high survival rate and low cost

Inactive Publication Date: 2012-10-17
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no practical guidance has been given on the operation methods and techniques for the establishment of the Taihangchrysanthemum aseptic vaccine system

Method used

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  • Tissue culture quick propagation method for Opisthopappus Shih
  • Tissue culture quick propagation method for Opisthopappus Shih
  • Tissue culture quick propagation method for Opisthopappus Shih

Examples

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Embodiment 1

[0032] The tissue culture rapid propagation method of daisy chrysanthemum comprises the following steps:

[0033] A. Explant acquisition:

[0034] A-1. Seed pretreatment: select chrysanthemum chrysanthemum seeds with full grains, soak the seeds in a sterile water bath at 37°C for 2h to 4h on an ultra-clean workbench, and use sterile absorbent paper to dry the surface of the material after the treatment ;

[0035] A-2. Inoculation culture: the seeds obtained in the previous step are inoculated on the inoculation medium, 10 seeds are inoculated in each inoculation bottle, and 2 to 4 true leaves grow in 30d to 40d; the composition of the inoculation medium is: MS culture The sucrose of the agar+3% weight fraction of the 6-BA+0.65% weight fraction of the NAA+2.0mg / l of base+0.2mg / l;

[0036] A-3. Selection and disinfection of explants: 35d to 45 days after inoculation, the largest leaves can grow to 2cm to 3cm. Pick the young leaves with light green color near the terminal buds,...

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Abstract

The invention discloses a tissue culture quick propagation method for Opisthopappus Shih, which comprises the following: A, a step of obtaining an explant, which is to obtain the explants from aseptic Opisthopappus Shih seedlings; B, a step of induction callus culture, which is to transfer the explants obtained by the step A to an induction callus culture medium and culture the explants till calla is generated; C, a step of propagation subculture, which is to inoculate calla that have a compact structure, fragile and hard texture and irregular spherical or nearly isodiametric cells, grow slowly and are light green onto a propagation subculture medium for subculture; D, a step of rooting culture, which is to transfer the aseptic seedlings which grow to more than 2 centimeters to a rooting culture medium for rooting culture; and E, a step of training and transplanting, which is to train the rooted seedlings at room temperature, transplant the seedlings into a medium and obtain a large number of Opisthopappus Shih seedlings. In the invention, detailed instructions are provided for building an Opisthopappus Shih seedling system, particularly, optimal formulae of the induction callus culture medium, propagation subculture medium and rooting culture medium are provided, the method have high actionability and repeatability and can propagate a large number of Opisthopappus Shih seedlings at one time.

Description

technical field [0001] The invention relates to a method for asexual propagation of plants, in particular to a method for rapid propagation of daisy chrysanthemum through tissue culture. Background technique [0002] Taihang chrysanthemum (Opisthopappus taihangensis (Ling) Shih) is a plant of the genus Opisthopappus chrysanthemum (Opisthopappus Shih), with a narrow distribution range and a unique living environment. Most of them grow in very steep cliff crevices that are difficult for people to reach. , only found in the Taihang Mountains at the junction of Henan, Shanxi, and Hebei provinces, and is a rare and endangered plant under national protection (see: Ding Baozhang, Wang Suiyi, Flora of Henan (Volume 3), Zhengzhou, Henan Science and Technology Press, J998.632). [0003] At present, there are only two reports on the tissue culture of Taihang Chrysanthemum, using stem segments and stem terminal buds as explants (Yao Lianfang, Department of Horticulture, Henan University...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄进勇苗一帆孙莉莉曾献秋
Owner ZHENGZHOU UNIV
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