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Method for extracting peanut peptide

An extraction method and peanut peptide technology, which is applied in the field of peanut peptide extraction and biocomposite enzyme enzymatic hydrolysis of peanut meal, can solve problems that threaten human health, and achieve the effects of no toxic side effects, small molecular weight, and high purity

Inactive Publication Date: 2011-02-09
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, a large amount of alkali treatment or some harmful chemical solvents are used in the extraction methods of peanut peptides, which inevitably have hidden dangers that threaten human health.

Method used

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Examples

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Embodiment 1

[0022] A method for extracting peanut peptides, the steps are as follows:

[0023] Commercially available peanut meal (protein content ≥ 46%) weighed 1kg, added 95g of 95wt% ethanol and 7kg of distilled water, stirred evenly, raised the temperature to 90°C and refluxed for 4h, filtered while hot after the reaction; then cooled the filtrate to 45°C, and used Adjust pH to 7-8 with 0.1M food-grade flake sodium hydroxide solution, add 40g of lipase, keep the temperature at 45°C, stir for 6 hours, and use 0.1M food-grade flake sodium hydroxide solution to keep the reaction during the enzymolysis Keep the pH of the system at 7-8; add 15g of alkaline protease, keep the temperature at 45°C, stir for 6 hours, and use 0.1M food-grade sodium hydroxide solution to keep the pH of the reaction system at 7. -8; then heat the obtained enzymolysis solution to 90°C and keep for 20min to inactivate the enzyme; then filter the enzymolysis solution to obtain a supernatant, add 20g of powdered acti...

Embodiment 2

[0028] A method for extracting peanut peptides, the steps are as follows:

[0029] Commercially available peanut meal (protein content ≥ 46%) weighs 2kg, adds 160g of 95wt% ethanol and 15kg of distilled water, stirs evenly, heats up to 95°C and refluxes for 3h, and filters while hot after the reaction; then cool the filtrate to 50°C, and use Adjust the pH to 7-8 with 0.1M food-grade flake sodium hydroxide solution, add 60g of lipase, keep the temperature at 50°C, stir for 5 hours, and use 0.1M food-grade flake sodium hydroxide solution to keep the reaction during the enzymolysis Keep the pH of the system at 7-8; add 10g of alkaline protease, keep the temperature at 50°C, and stir for 8 hours for enzymolysis. During the enzymolysis, use 0.1M food-grade sodium hydroxide solution to keep the pH of the reaction system at 7 -8; then heat the obtained enzymolysis solution to 95°C and keep it for 15min to inactivate the enzyme; then filter the enzymolysis solution to obtain a superna...

Embodiment 3

[0034] A method for extracting peanut peptides, the steps are as follows:

[0035] Weigh 10 kg of commercially available peanut meal (protein content ≥ 46%), add 1 kg of 95 wt% ethanol and 90 kg of distilled water, stir evenly, heat up to 93 ° C and reflux for 3.5 h, filter while hot after the reaction; then cool the filtrate to 55 ° C, Adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution in flake form, add 400g of lipase, keep the temperature at 55°C, and stir for 4.5 hours for enzymolysis. During the enzymolysis process, use 0.1M food-grade sodium hydroxide solution in flake form Keep the pH value of the reaction system at 7-8; add 100g of alkaline protease, keep the temperature at 55°C, stir for 7 hours, and use 0.1M food-grade sodium hydroxide solution to maintain the pH value of the reaction system during the enzymolysis process In 7-8; then heat the obtained enzymolysis solution to 100°C and keep it for 10 minutes to inactivate the enzyme; then filter the ...

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Abstract

The invention discloses a method for extracting peanut peptide, which takes peanut cake as a raw material, and comprises the following steps: adding water and ethanol to the peanut cake, mixing, heating, refluxing, filtering, degreasing lipase, carrying out enzymatic hydrolysis on protease, inactivating enzyme, decolorizing, separating membrane, and drying obtained filtrate to obtain the peanut peptide. The method of the invention takes the byproduct peanut cake discarded in peanut processing as the raw material, obtains the peanut peptide by a biological method, has short production cycle, low cost, simple operation and mild reaction conditions, and does not generate any toxic harmful substances, and the obtained product has high safety and can be widely applied to the fields of medicine, health products, biosynthesis and the like.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of peanuts, in particular to a method for extracting peanut peptides, which is a method for extracting peanut peptides by using peanut meal as a raw material to enzymatically hydrolyze peanut meal with biological compound enzymes. Background technique [0002] The geographical distribution of peanut planting in my country is very wide, from Hainan Island in the south to Heilongjiang in the north, from Taiwan in the east to Xinjiang in the west, but mainly concentrated in Shandong, Henan, Hebei and other provinces, accounting for more than 60% of the national peanut production. In normal years, my country's peanut production is about 10 million tons, and my country's peanut production ranks first in the world, accounting for 38% of the world's total production. Peanut meal is a product obtained by pressing peanut kernels and extracting oil. Peanut meal is divided into primary ...

Claims

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Application Information

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IPC IPC(8): A23J3/14A23J3/34
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司
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