Method for extracting shikonin from lithospermum

Abstract

The invention relates to a method for extracting shikonin from lithospermum, which comprises the following steps of: crushing lithospermum raw materials and adding alkaline aqueous solution with the pH value being 9 to 10 for percolating and extracting; regulating the pH of the extracting solution to 7, 5 and 3, adding a macroporous resin column to adsorb for three times, eluting by using 70 to 80-percent ethanol, concentrating the eluent and crystallizing in ethanol; dissolving in sodium bicarbonate solution with the pH value being 10 to 11, regulating the pH value to 1 to 3 and precipitating, and refluxing and dissolving the precipitates in acetone and ethyl acetate in turn and recrystallizing. The method for producing the shikonin can reduce the use amount of toxic reagent and reduce production cost. The process is simple in operation and easy in industrialization.

Description

Technical field:

[0001] The invention relates to a method for extracting shikonin from shikonin, in particular to a method for purifying shikonin with macroporous resin. Background technique:

[0002] Shikonin naphthoquinone compounds, also known as shikonin, shikonin, shikonin.

[0003] Molecular formula: C 16 h 16 o 5 ;

[0004] Molecular weight: 288.31

[0005] Molecular formula:

[0006]

[0007] Physical and chemical properties: purple flaky crystal or crystalline powder. The melting point is 147-149°C. Optical rotation +138° (benzene). Insoluble in water, soluble in ethanol, organic solvents and vegetable oils. It is easily soluble in alkaline water, and precipitates out when it encounters acid.

[0008] Shikonin has anti-cancer, anti-inflammatory, antibacterial and other effects. It is clinically used to treat acute and chronic hepatitis and liver cirrhosis (ascites). Dermatology is used for the treatment of flat warts, psoriasis, local application of b...

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