Method for quantitatively detecting toxigenic microcystis and special standard substance thereof

A technology of microcystin and standard products, which is applied in biochemical equipment and methods, measurement/inspection of microorganisms, introduction of foreign genetic material using carriers, etc., can solve the problems that microcystins cannot meet early warning and water quality safety control, etc. Achieve the effects of wide linear detection range, good purity, and simple preparation method
CN101974632AInactive Publication Date: 2011-02-16TSINGHUA UNIV
0 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for quantitatively detecting toxigenic microcystis and a special standard substance thereof. The standard substance for detecting the toxigenic microcystis in a sample to be detected is recombinant plasmid or recombinant bacterium or recombinant cell which contains mcyA gene; and the sequence of the mcyA gene is sequence 1 in the sequence list. The departure plasmid of the recombinant plasmid is pMD-18T.The standard substance has the characteristic of combination of broad spectrum identification and specificity, high sensitivity, simple preparation method, long retention period, good purity and wide linear detection range, thus being capable of being applied to fast quantitative detection of the toxigenic microcystis in a sample in the water environment.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for quantitatively detecting toxin-producing Microcystis and a special standard product thereof. Background technique

[0002] Microcystins (MCs for short) are monocyclic heptapeptides produced by cyanobacteria (cyanobacteria), especially some Microcystis (Microcystis), and are commonly found in water bodies where cyanobacteria blooms occur. Microcystin is a liver toxin, long-term drinking of water containing microcystin can cause liver damage and even liver cancer.

[0003] Microcystins are a class of toxins that are regulated by toxin-producing genes and metabolized in cells. Studies have shown that not all Microcystis can produce microcystins, and the toxicity of Microcystis strains (Toxic strains) and nontoxic strains (Nontoxic strains) is determined by genetics, and there is no obvious difference in morphology. difference. Microcystins are non-ribosomally synthesized by the peptide synthase complex gene cluster...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More