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Preparation consisting of PLGA (Polylactic Acid-Polyglycolic Acid)/TCP (Tris Calcium Polymer) and MSC (Mesenchymal Stem Cells) of compound MSC and application thereof

A preparation and polymer technology, which is applied to a preparation composed of PLGA/TCP and MSC composited with MSC and its application field, can solve problems such as success rate, difficult emergency surgery, and increased body trauma.

Inactive Publication Date: 2011-03-30
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings. The current research on using MSCs to promote tissue engineering bone is basically autologous cell transplantation, which has many limitations: limited source of autologous MSCs; increased body trauma; in vitro expansion takes a certain time and is difficult to apply. emergency surgery, etc.
However, there is no report on the immune response caused by bioengineered bone in vivo, and the success rate of bioengineered bone transplantation is also a potential problem

Method used

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  • Preparation consisting of PLGA (Polylactic Acid-Polyglycolic Acid)/TCP (Tris Calcium Polymer) and MSC (Mesenchymal Stem Cells) of compound MSC and application thereof
  • Preparation consisting of PLGA (Polylactic Acid-Polyglycolic Acid)/TCP (Tris Calcium Polymer) and MSC (Mesenchymal Stem Cells) of compound MSC and application thereof
  • Preparation consisting of PLGA (Polylactic Acid-Polyglycolic Acid)/TCP (Tris Calcium Polymer) and MSC (Mesenchymal Stem Cells) of compound MSC and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of biomaterial composite bone chips

[0030] 1. Aseptic treatment of biological materials

[0031] The biological material polylactic acid polyglycolic acid / tricalcium phosphate polymer (PLGA / TCP, number average molecular weight is 30,000, Chengdu Organic Chemistry Co., Ltd., Chinese Academy of Sciences) is all cut into small pieces (each specification is 5mm long, 5mm wide, 1-2mm thick), soak in 70% ethanol for half an hour to make the material hydrophilic, then rinse with PBS several times, sterilize with ultraviolet light for half an hour, and finally fully soak in α-MEM (Gibco) + 10% FCS (Hyclone) .

[0032] 2. Isolation and culture of MSCs from mouse bone parenchyma

[0033] 2-3 weeks old C57BL / 6 mice (purchased from the Animal Center of the Academy of Military Medical Sciences), the mice were killed by neck dislocation, the mice were soaked in 75% alcohol to disinfect the mice → the mouse tibia and femur were separated under aseptic conditions, an...

Embodiment 2

[0067] The difference between this example and the example is that the isolated MSCs were replaced with commercial MSCs (C3H10T1 / 2, Shanghai Fuyi Medical Research Service Center), and the rest of the experiments were identical. The results showed that there was no significant difference between the results of this example and Example 1, indicating that the intravenous infusion of mesenchymal stem cells can reduce the immune response caused by the allograft of biomaterials or biomaterial composite bone chips.

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Abstract

The invention discloses a preparation consisting of a PLGA (Polylactic Acid-Polyglycolic Acid) / TCP(Tris Calcium Polymer) and MSC (Mesenchymal Stem Cells) of a compound MSC and application thereof. Active components of the preparation comprise a substance (1) and a substance (b) which are separately packed, wherein the substance (1) is a biological compound sclerite compounded by the MSC and the PLGA / TCP; and the substance (2) is the MSC. Proved by experiments, the intravenous infusion of the substance (2), i.e. the MSC, can be used for lightening immune reactions caused by the heteroplastic transplantation of the compound sclerite.

Description

technical field [0001] The present invention relates to a preparation composed of PLGA / TCP and MSC compounded with MSC and its application. Background technique [0002] Bone and cartilage defects caused by diseases and traumas are common clinical and difficult to treat diseases, and articular cartilage defects caused by various reasons and lack of effective repair ability often cause pain and joint dysfunction after injury. . Mesenchymal stem cells offer broad prospects for the treatment of bone defects. [0003] Mesenchymal stem cells (MSCs) have a wide range of sources, widely exist in connective tissue and organ interstitium throughout the body, have self-renewal and proliferation capabilities, and have multipotential differentiation characteristics in a suitable microenvironment. Based on the advantages of easy acquisition, easy culture, convenient transportation, low immunogenicity, immunoregulatory properties, long-term survival in the host body, easy transfection o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/18A61L27/38A61K35/12A61P37/06
Inventor 毛宁孔维霞张毅李红朱恒江小霞刘元林吴英
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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