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Extracting and purifying method for sapindus mukorossi gaerth total saponin

A technology for total saponins and Sapindus japonicas, applied in the field of extraction and purification of Sapindus japonica total saponins, can solve the problems of poor operability, high production cost and high cost, and achieve the effects of reducing volume, improving purity and increasing purity

Active Publication Date: 2011-11-09
FUJIAN YUANRONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wei Fengyu et al. used water extraction-macroporous resin adsorption separation method and ultrafiltration method to separate and purify the total saponins of Sapindus chinensis. The purity of the total saponins can reach 85% and 67.2% respectively, but the cost of industrial production is high
In summary, although researchers have done a lot of work on the separation and extraction of Sapindus chinensis, they are still in the preliminary research stage in general, and there are still problems of low purity (such as water extraction) or It is the disadvantages of high cost and poor operability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1, Sapinberry fruit was dried in an oven at 60°C, peeled, and crushed to 20 meshes. Take 10 Kg of Sapinberry peel powder and put it into a 100L extractor, add 20 L of water, and stir under normal pressure and 30°C constant temperature After 2 hours, soak and extract twice, combine the extracts and centrifuge to obtain low-purity extracts. Sterilize at 72°C for 15 seconds, inoculate 0.1% Aspergillus oryzae in the extract, culture at constant temperature and constant temperature at 30°C under normal pressure for 3 days, and centrifuge to obtain the supernatant. The supernatant was dehydrated with a spray dryer to obtain 3.8 Kg of sapindus total saponins powder product, the extraction rate of total saponins was 18%, and the purity of total saponins was 50%.

Embodiment 2

[0018] Example 2 Sapindus fruit was dried in an oven at 60°C, peeled, and crushed to 5 meshes. Take 10 Kg of Sapindus fruit peel powder and put it into a 100L extractor, add 40 L of water, and stir for 7 hours at normal pressure and 40°C. , soak and extract twice, combine the extracts and centrifuge to obtain low-purity extracts. Sterilize at 72°C for 15 seconds, cool down, inoculate 0.2% Aspergillus oryzae in the extract, culture at 25°C for 15 days with constant temperature shaking, and centrifuge to obtain the supernatant. The supernatant was dehydrated with a spray dryer to obtain 3.8 Kg of sapindus total saponins powder product, the extraction rate of total saponins was 20%, and the purity of total saponins was 55%.

Embodiment 3

[0019] Example 3 Soapberry fruit was dried in an oven at 60°C, peeled, and crushed to 10 meshes. Take 10 Kg of Sapinberry peel powder and put it into a 100L extractor, add 30L of water, and stir at a constant temperature of 50°C under normal pressure for 8 hours , soaked and extracted three times, combined extracts and centrifuged to obtain low-purity extracts. Sterilize at 72°C for 15 seconds, cool down, inoculate 0.3% yeast in the extract, culture at 30°C for 25 days with constant temperature shaking, and centrifuge to obtain the supernatant. The supernatant was dehydrated with a spray dryer to obtain 3.8 Kg of sapindus total saponins powder product, the yield of total saponins was 22%, and the purity of total saponins was 60%.

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Abstract

The invention relates to extraction of active ingredients of plant fruits, in particular to an extracting and purifying method for sapindus mukorossi gaerth total saponin. The method mainly comprises the following processing steps: drying sapindus mukorossi gaerth peel, smashing, adding water at a proper temperature and extracting for 1 to 24 hours, wherein the yield of saponin is 15-25%, and thegrade of purity is 15-25%; sterilizing the extracting solution at 72 DEG C for 15 seconds and cooling; vaccinating 1-5% yeast strain or aspergillus oryzae, adjusting to a proper temperature, and oscillating or standing and culturing for 2-30 days, wherein the microorganism propagates by using the sugar in the extracting solution, but the notoginsenosides content is basically unchanged; and centrifuging, thus obtaining a medium-purity extracting solution, wherein the yield of the saponin is 15-25%, and the degree of purity is 45-60%. The sapindus mukorossi gaerth total saponin production process provided by the invention is high in extraction efficiency, simple in operation and low in cost, is healthy and non-toxic, can be used in cleaning products and cosmetics with different requirements, and has wide application range and higher economic benefit.

Description

technical field [0001] The invention relates to the extraction of effective components of plant fruits, in particular to a method for extracting and purifying total sapindus sapinsin. Background technique [0002] Sapindus mukorossi Gaerth., also known as soap tree or hand-washing fruit, is a deciduous tree of Sapindus family Sapindus. It is distributed in Southeast Asian countries, Taiwan Province of my country and the provinces south of the Huaihe River. Sapindus aril is rich in glycosides, mainly triterpene saponins and sesquiterpene glycosides, which have good foaming and decontamination properties, and can be used as natural active substances in shampoos and various It also has physiological effects such as antibacterial and antipruritic, and can be used for the treatment of athlete's foot; studies such as Ojha have shown that compared with artificially synthesized nonionic cleansers such as N-9, Sapindus total saponins have Lactobacillus has low toxicity and can be us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/97A61Q19/10A61Q19/00A61Q5/02
Inventor 翁震郭有枝张翠汪何雅姚卫蓉
Owner FUJIAN YUANRONG BIOTECH
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