Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, screening method and application thereof

A mononuclear cell proliferation and nucleic acid aptamer technology, applied in biochemical equipment and methods, microbial measurement/testing, DNA preparation, etc., can solve time-consuming and difficult problems, and achieve low preparation cost, single-stranded oligonuclear The effect of stable glycoside acid and easy operation

Active Publication Date: 2011-04-13
JIANGNAN UNIV
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In essence, most of the surface antigens contained in the same genus are the same, with only slight differences. It is obviously a time-consuming and arduous task to find these differences and prepare corresponding specific antibodies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, screening method and application thereof
  • Nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, screening method and application thereof
  • Nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The following is the preparation method of nucleic acid aptamers that specifically bind to Listeria monocytogenes by SELEX technology and its application in the rapid detection of Listeria monocytogenes.

[0017] 1. Synthesize random single-stranded DNA library and primers (synthesized by IDT)

[0018] Random single-stranded DNA (ssDNA) library: 5′-GGGAGCTCAGAATAAACGCTCAA(N35)TTCGACATGAGGCCCGGATC-3′, a random ssDNA library with a length of 78 nt was constructed, with fixed primer sequences at both ends and a random sequence of 35 bases in the middle, the library capacity for 10 14 Above; Primer I: 5′-GGGAGCTCAGAATAAACGCTCAA-3′; Primer II: 5′-GATCCGGGCCTCATGTCG AA-3′, the random ssDNA library and the two primers were made into 100 μmol / L stock solution with TE buffer and stored at -20°C for later use .

[0019] 2. PCR amplification of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) libraries

[0020] Before each round of screening, the ssDNA library was am...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, a screening method and application thereof. By using the SELEX (Systematic Evolution of Ligands By Exponential Enrichment) technology, a single-chain DNA oligonucleotide aptamer capable of specifically recognizing Listeria monocytogenes is obtained, and the aptamer can be transformed into a reporting aptamer by means of fluorescein labels and the like to be used for detecting Listeria monocytogenes. The sequence of the aptamer has wide application prospects in the aspect of accurately and fast detecting Listeria monocytogenes in food.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation of a nucleic acid adapter with high specificity and high affinity for Listeria monocytogenes by using the SELEX technology (exponentially enriched ligand system evolution technology) in molecular biology technology. The gamete provides a scientific basis and a theoretical basis for the application of the nucleic acid aptamer in the detection of Listeria monocytogenes. Background technique [0002] Listeria monocytogenes is a common and important zoonotic pathogen. It can cause listeriosis in humans and animals. After severe infection, it mainly manifests as shortness of breath, vomiting, hemorrhagic rash, suppurative conjunctivitis, fever, convulsions, coma, spontaneous abortion, meningitis, sepsis and even death. It exists widely in nature. Listeria monocytogenes in food is dangerous to human safety. The bacteria can still grow and reproduce in 4°C environment, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115C12N15/10C12Q1/68C12Q1/04
Inventor 王周平丁晓莹
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com