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Pseudomonas sp. XQ23 capable of efficiently degrading multiple phenolic compounds

A technology for phenolic compounds and Pseudomonas, which can be used in microorganism-based methods, bacteria, microorganisms, etc., and can solve the problems of few research reports on the degradation of phenolic compounds.

Inactive Publication Date: 2011-04-27
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For a long time, there have been many reports on the breeding and research of phenol-degrading bacteria at home and abroad, but there are few research reports on the degradation of most other phenolic compounds of the present invention, especially the simultaneous degradation of up to ten kinds of phenolic compounds with the same strain of bacteria The invention is still the first case at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Strain enrichment screening and isolation and purification

[0012] 1.1 Preparation of enrichment medium

[0013] According to the following formula: Each liter of deionized water contains: 200 mg 2,3-dimethylphenol, 2.28 g K 2 HPO 4 ·3H 2 O, 0.47 g NaH 2 PO 4 2H 2 O, 1.32 g (NH 4 ) 2 SO 4 , 0.12 g MgSO 4 , 2.63 mg CaCl 2 , 0.72 mg FeSO 4 ·7H 2 O, 0.46 mg ZnSO 4 ·7H 2 O, 0.22 mg MnSO 4 ·H 2 O, 0.50-0.00 grams of yeast powder. Adjust the pH to 7.0, and sterilize at 121°C for 20 minutes. Among them, 0.50-0.00 gram of yeast powder refers to: 0.50 gram is added at the first enrichment, and then gradually decreased to 0.00 gram at the fourth time.

[0014] 1.2 Strain enrichment

[0015] Weigh 5 grams of sludge heavily polluted by phenols, inoculate it into a shaker flask containing 100 ml of the above-mentioned enrichment medium, place it in a shaker with a rotation speed of 150 rpm, and control the temperature at 30°C. About 3 days later, take...

Embodiment 2

[0019] Embodiment 2: strain identification and preservation

[0020] 2.1 Morphological characteristics

[0021] The colonies are milky white, smooth, with neat edges. The bacteria were rod-shaped and Gram staining was negative.

[0022] 2.2 Identification and preservation of strain 16S rDNA

[0023] Using bacterial 16S rDNA universal primers 27F and 1387R as amplification primers, the 16S rDNA fragment of the target strain obtained in the above 1.3 experiment was amplified by PCR method, and after electrophoresis detection, it was sent to Shanghai Sangon Bioengineering Technology Service Co., Ltd. Sequencing results, and using the BLAST program of the National Center for Biotechnology Information (NCBI) for nucleotide homology comparison between the 16S rDNA sequence of the bacteria and the sequence already included in GenBank, it was found that the sequence homology with it was greater than 99% The known strain is Pseudomonas (Pseudomonas sp.), so the strain of the present...

Embodiment 3

[0031] Embodiment 3: prepare Pseudomonas XQ23 bacterial agent

[0032] Pseudomonas XQ23 described in Example 2 was inoculated in a shaker flask with a liquid volume of 50 milliliters, and cultivated in a constant temperature shaker. The medium used is LB medium; the culture conditions are: temperature 30°C, shaker speed 150 rpm. When the cell growth reaches the end of logarithmic growth, that is, the culture is generally about 12 hours, the culture is stopped, and the culture fluid is harvested to obtain the liquid bacterial agent. The bacterial agent can be used directly, or placed in a refrigerator at 4°C for use. The formula of the LB medium is: every liter of deionized water contains: 10 grams of tryptone, 5 grams of yeast extract, and 10 grams of sodium chloride. Adjust the pH to 7.0, and sterilize at 121°C for 20 minutes.

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Abstract

The invention discloses a pseudomonas sp. XQ23 capable of efficiently degrading multiple phenolic compounds. The strain of the pseudomonas sp. XQ23 is separated and purified from sludge which is seriously polluted by phenols. The strain has been preserved in China Center for Type Culture Collection since August 8th, 2009, and the preservation No. is CCTCC M 209175. The invention belongs to the technical field of environmental protection, and especially aims to the degradation problem of phenolic compounds in phenol-containing sewage generated in the petroleum refining process. Ten phenolic compounds, such as phenol, methyl phenol and the like, generally account for more than 97% of the total phenol content in the phenol-containing sewage. The strain is inoculated to the water body which contains one, or some or all of the ten phenolic compounds, and necessary inorganic salts are added; and while controlling the reaction temperature at 30+ / -2 DEG C and the controlling initial pH value to 7.0+ / -0.2 during degrading, the strain can completely degrade the phenolic compounds within shorter time under aerobic conditions.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a strain of Pseudomonas XQ23 which efficiently degrades various phenolic compounds and a method for degrading the phenolic compounds in phenol-containing sewage produced in the petroleum refining process. Background technique [0002] Phenolic compounds are an important class of chemical raw materials, which are widely used in chemical manufacturing industries such as resins, plastics, dyes, medicines, and pesticides. Phenolic compounds are also the most important organic components in the phenolic sewage of the petroleum refining industry. According to research, the phenolic substances in the sewage produced by a petrochemical plant through coking, catalytic cracking, atmospheric and vacuum and other petroleum refining processes accounted for the total amount of organic matter respectively. 97.53%, 92.95% and 90.94%, and the following ten phenolic co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/34C12R1/38
Inventor 肖梓军亓瑞倩乔树苓严勇徐海吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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