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Microbial live bacterium agent, preparation method and application thereof

A technology of live bacteria preparation and microorganism, applied in the field of microorganism preparation, can solve problems such as being unfavorable to human health, unable to guarantee vitality, and reducing the number of live bacteria, and achieve the effects of comprehensive health care efficacy, low cost and wide applicability

Active Publication Date: 2014-04-30
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned microbial preparations use milk powder, glucose, etc. as microbial freeze-drying protective agents, which are costly and contain animal-derived ingredients, which are not conducive to human health and are not suitable for specific groups of people.
In addition, although it can protect microorganisms to a certain extent, the number of viable bacteria decreases rapidly during a longer storage period, and its vitality cannot be guaranteed after long-term storage

Method used

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  • Microbial live bacterium agent, preparation method and application thereof
  • Microbial live bacterium agent, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of live lactic acid bacteria preparation:

[0025] Take the prepared bacterial cellulose membrane (also commercially available), cut it into 5×5mm cubic particles, sterilize and reserve;

[0026] Take 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, Tween 801ml, 2g diammonium hydrogen citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, MnSO 4 ·7H 2 O0.05g, MgSO 4 ·7H 2 0.2g of O, 20g of calcium carbonate, 20g of agar, and 1L of distilled water were used to prepare a culture medium, adjusted to pH 6.8 with NaOH, and sterilized.

[0027] Add the above bacterial cellulose particles to the culture medium at a volume ratio of 30:100, and stir and mix;

[0028] Inoculate Lactobacillus bulgaricus and cultivate for 12 hours at 37°C;

[0029] Take out the bacterial cellulose particles under aseptic conditions and wash them with sterile distilled water;

[0030] Use a freeze dryer at -40°C and a vacuum of 6Pa for 12 hours;

[0031] The powdered lactic acid...

Embodiment 2

[0033] Preparation of live bifidobacterium preparation:

[0034] Take the prepared bacterial cellulose membrane, cut it into 3×3mm cubic particles, sterilize and reserve;

[0035] Take the commercially available Bifidobacterium BS medium (Shanghai Hufeng Biotechnology Co., Ltd.) and sterilize;

[0036] Add the above-mentioned bacterial cellulose particles to the culture medium at a volume ratio of 50:100, and stir and mix;

[0037] Inoculate Bifidobacterium bifidum and cultivate for 10 hours at 37°C;

[0038] Take out the bacterial cellulose particles under aseptic conditions and wash them with sterile distilled water;

[0039] Pre-cooling at -10℃ for 15min;

[0040] Use a freeze dryer at -80°C and a vacuum of 40 Pa for 8 hours;

[0041] Crushing and aseptically packaging to prepare a powdered live bifidobacterium preparation.

Embodiment 3

[0043] Preparation of live yeast preparations:

[0044] Take the prepared bacterial cellulose membrane, cut it into 8×8mm cubic particles, sterilize and reserve;

[0045] Take the commercially available yeast YPD medium (Shanghai Jiemei Gene Pharmaceutical Technology Co., Ltd.) and sterilize;

[0046] Add the above-mentioned bacterial cellulose particles to the culture medium at a volume ratio of 70:100, and stir and mix;

[0047] Inoculate Saccharomyces cerevisiae and cultivate for 5 hours at 27°C;

[0048] Take out the bacterial cellulose particles under aseptic conditions and wash them with sterile distilled water;

[0049] Pre-cooling at -20℃ for 8min;

[0050] Use a freeze dryer at -100°C and a vacuum of 60 Pa for 7 hours;

[0051] Crush, aseptically package to prepare a powdered yeast preparation.

[0052] Detection:

[0053] The live bacteria preparations in Examples 1 to 3 were respectively taken and stored at 4°C. The number of viable bacteria was determined, and the results are sh...

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Abstract

The invention belongs to the field of microbial agents, and in particular relates to a microbial live bacterium agent and a preparation method thereof. The microbial live bacterium agent is prepared by a microbial agent preparation process and by taking bacterial cellulose as a freeze-drying protective agent and carrier; the preparation method for the microbial live bacterium agent comprises the following steps of: preparing the bacterial cellulose into particles, adding the particles into a microbiological culture medium, uniformly stirring and mixing the particles, and then inoculating microorganisms and culturing; separating the bacterial cellulose particles under the aseptic condition, and washing the bacterial cellulose particles with sterile distilled water; and freezing and drying the bacterial cellulose particles, then crushing the particles into powder, and finally packaging the powder under the aseptic condition. The microbial live bacterium agent provided by the invention can effectively protect the activity of lactic acid bacteria; and the microorganisms still have high activity. The microbial live bacterium agent has the characteristics of convenience for use, low cost, wide applicability and the like, can be widely applied in food industry, and has more complete healthcare effects.

Description

Technical field [0001] The invention belongs to the field of microbial preparations, and specifically relates to a viable microbial preparation and a preparation method thereof, and its application in the food field, including a composite viable microbial preparation containing the viable microbial preparation. Background technique [0002] Microorganisms are tiny, but they are closely related to human life. Many microorganisms are also used in the production of food, such as brewing, vinegar making, and producing bread, dairy products and beverages. At present, the use of food microorganisms can be roughly divided into two categories. One is the use of microorganisms to ferment to produce food, such as brewing beer with beer yeast, and the other is to use microorganisms to improve the host's microecological balance and play a beneficial role. The characteristics are directly added to various foods, such as the use of probiotics. When using microorganisms, the activity of the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12
Inventor 钟春燕
Owner 钟春燕