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Method for making once-forming tissue chip and mold applied to same

A technology of tissue chips and molds, which is applied in the field of making one-time forming tissue chips and molds. It can solve the problems of complicated tissue chips, excessive wax melting, and high technical requirements, so as to improve production speed and quality, reduce displacement and chip loss rate, The effect of simplifying the production process

Inactive Publication Date: 2011-05-04
浙江省台州医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The application prospect of tissue chip is very broad, but the production of tissue chip is very cumbersome. It needs to make hollow wax model, secondary embedding, etc., and tissue chip production equipment with vacuum function is also required.
Moreover, the production process requires high technology, and it is prone to deformation or cracking when making hollow wax models; poor tissue core fusion or excessive wax melting are prone to occur during secondary embedding; question
At present, the price of more advanced tissue microarray instruments is as high as hundreds of thousands of RMB, and even domestically produced ones cost tens of thousands of RMB, and they are all very cumbersome.
These shortcomings have greatly restricted the development and application of tissue chips, and made it impossible to promote tissue chip technology in grassroots hospitals or scientific research units.

Method used

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  • Method for making once-forming tissue chip and mold applied to same
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  • Method for making once-forming tissue chip and mold applied to same

Examples

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Effect test

Embodiment 1

[0034]In the present invention, the mold for making a one-time forming tissue chip includes an array hole copper plate 1, an array needle copper plate 2 and two L-shaped copper spacers 3, and the array hole copper plate 1 is provided with n×n rectangular array through holes 4, and the array through hole The size and arrangement of 4 can be flexibly changed as required. One side of the array needle copper plate 2 is provided with an array needle 5 corresponding to the array through hole 4 one by one. The arrangement of the array needle 5 is exactly the same as that of the array through hole 4. The same as the depth, the array hole copper plate 1 and the array needle copper plate 2 are rectangular, and both have the same width, and the length of the array needle copper plate 2 is slightly longer than the length of the array hole copper plate 1, which is convenient for assembly and disassembly. Take the 6×7 array as an example to provide reference data: the number of array throug...

Embodiment 2

[0046] The content of embodiment 2 is basically the same as that of embodiment 1, the difference is that the mold uses two types of L-shaped copper pads 3, in addition to the 1mm thick copper pad in embodiment 1, there is also a 0.2mm thick copper pad piece. Since in Example 1, the operation of jacking up the tissue core needs to be carried out after the paraffin has solidified slightly, resulting in some tiny air bubbles remaining between the tissue core and the wax body in the jacked-up part, although the air bubbles do not affect the stability of the tissue core, nor affect the stability of the tissue core. Sectioning and staining, but it affects the beauty of the tissue microarray wax block. Therefore, different from Example 1 in the steps of preheating and wax injection, in this embodiment, the L-shaped copper spacer 3 with a thickness of 1 mm and the L-shaped copper spacer 3 with a thickness of 0.2 mm are prepared first, and after filling the tissue core Remove the 1mm ...

Embodiment 3

[0049] Embodiment 3 is basically the same as Embodiment 2, except that it is different from Embodiment 2 in the steps of preheating and wax injection. The 1mm thick L-shaped copper gasket 3 removed after filling the tissue core is replaced with a 0.2mm L-shaped copper pad 3. Copper spacer 3, push up the tissue core evenly, place the wax bearing tank 6 in the mold on the array hole copper plate 1, so that the array cavity 7 is located in the wax bearing tank 6, and then put the mold as a whole into the incubator for pre-heating. Heat until the wax column is slightly melted—the surface is shiny; take the mold out of the incubator, and immediately inject paraffin wax at 70°C, the amount injected should not exceed the supporting surface of the plastic embedding box, and wait for the paraffin wax in the wax holding tank 6 After the solidification is no longer transparent, put it into a plastic embedding box, and then fill it with 70°C paraffin.

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Abstract

The invention provides a method for making a once-forming tissue chip and a mold applied to the same, belonging to the technical field of tissue chip making and solving the problems of high technical requirements and the like in the traditional tissue chip making process. The method comprises the following steps of: firstly, assembling a mold; secondly, taking a core and filling; thirdly, preheating and injecting wax; fourthly, disassembling; and fifthly, slicing and dyeing. The method can be used for making the once-forming tissue chip, simplify the making flow and has the advantages of simple operation steps, rapidness, economy, easiness of use and the like.

Description

technical field [0001] The invention belongs to the technical field of making tissue chips, and relates to a method for making one-time forming tissue chips and a mold used in the method. Background technique [0002] Tissue chip (tissue chip), also known as tissue microarray (tissue microarray, TMA), is a tissue section made by arranging dozens to thousands of small tissues neatly on a glass slide. It is another important biochip after gene chips and protein chips. It is mainly used to study the expression of the same gene or protein molecule in different cells or tissues. It has high throughput, large samples, time-saving and fast Etc. Since Kononen et al. put forward the clear concept of tissue chip and confirmed its function in 1998, tissue chip technology has developed rapidly. This technology has been applied in human genome research, medical diagnosis and basic research, especially in tumor gene screening, tumor antigen screening and finding markers related to tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/44
Inventor 林爱芬包卫光颜卫华
Owner 浙江省台州医院
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