Infectious bovine rhinotracheitis vaccine and preparation method thereof

A technology for rhinotracheitis and vaccine preparation, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc.

Inactive Publication Date: 2011-05-11
TIANJIN RINGPU BIO TECH
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a vaccine for bovine infectious rhinotracheitis and its preparation method, which is used to solve the problem of vaccine prevention and control of regional epidemics of bovine infectious rhinotracheitis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Infectious bovine rhinotracheitis vaccine and preparation method thereof
  • Infectious bovine rhinotracheitis vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 bovine infectious rhinotracheitis vaccine preparation method

[0023] 1. Virus isolation: Aseptically collect the liver, spleen, heart, kidney, placenta, and lung of IBR aborted fetuses, rinse with sterilized Hanks solution several times, cut into pieces, and grind. Then add 5 times the amount of Hanks (containing 200IU / ml penicillin and 200μg / ml streptomycin) to make an emulsion, transfer it into a 10ml centrifuge tube, and freeze and thaw repeatedly three times. Centrifuge at 3000r / min for 10min, take the supernatant and filter to sterilize. Dilute 10 times, 100 times, 1000 times with Hanks solution, and store at -20°C for later use. Select the calf testicular primary cells that grow into a dense monolayer and intact form, discard the nutrient solution, wash the cells with Hanks solution for 3 times, and add the treated diseased cells according to four gradients of stock solution, 10 times, 100 times and 1000 times respectively. Feed supernatant, the am...

Embodiment 2

[0033] Embodiment 2 immune effect test

[0034] 1. Materials

[0035] 1.1 Bovine infectious rhinotracheitis oil emulsion inactivated vaccine

[0036] 1.2 Bovine infectious nasal and tracheal antibody-negative adult dairy cows

[0037] 2. Method

[0038] A total of 70 adult dairy cows negative for bovine infectious nasal and tracheal antibodies were selected and randomly divided into 4 groups, with 3 groups as the immunization group and 1 group as the control group. The immunization group was injected intramuscularly with 1 portion, 2 portions, and 4 portions of the bovine infectious rhinotracheitis oil emulsion inactivated vaccine prepared in Example 1 respectively; the control group was not immunized, and blood was collected to measure antibodies respectively 35 days after immunization. Grouping and immunization methods are shown in Table 1.

[0039] Table 1 Grouping and immunization methods

[0040]

[0041] 3. Results and Analysis

[0042] 3.1 Serum test results ar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an infectious bovine rhinotracheitis vaccine and a preparation method thereof, belongs to the technical field of veterinary biological products, and is used for solving the problem to control the endemic infectious bovine rhinotracheitis by a vaccine. The vaccine is prepared by the following steps of: separating virus from a tissue of aborted fetus of cow infected with the infectious bovine rhinotracheitis; breeding; detecting titer; inactivating and emulsifying and the like. The vaccine can be used for controlling the endemic infectious bovine rhinotracheitis.

Description

technical field [0001] The invention relates to a bovine infectious rhinotracheitis vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. Background of the invention [0002] Infectious Bovine Rhinotracheitis (IBR) is an acute, febrile, contact infectious disease that occurs in cattle caused by Infectious Bovine Rhinotracheitis virus (IBRV). Difficulty, rhinitis, sinusitis, and upper airway inflammation are characterized. It can also cause damage to the reproductive system of cows, miscarriage and stillbirth, as well as enteritis and calf encephalitis, and sometimes conjunctivitis and keratitis. It has a greater impact, and acute IBR respiratory infection can be secondary to fatal bacterial pneumonia in cattle, which is one of the main reasons for the economic loss of the cattle industry. [0003] In recent years, with the rapid increase in the number of dairy cows, the number of abortions in the herd has increased, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/265A61P31/20
Inventor 马丽伟申宏旺夏雪林刘爱玲
Owner TIANJIN RINGPU BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap