Ion chromatography-electrochemical derivative-fluorescence analysis system

Abstract

The invention relates to an ion chromatography-electrochemical derivative-fluorescence analysis system which comprises a liquid chromatography pump, a sample introduction valve, an ion chromatography column, a self-made electrolytic cell, a power supply and a fluorescence detector. A polar organic compound without or with weak fluorescence intensity and a homologous compound thereof are separated on the ion chromatography column, separated components are oxidized to a strong fluorescence material on line in an anode chamber of the self-made electrolytic cell, and the strong fluorescence material is detected and analyzed by the fluorescence detector. Tail liquid flowing from the fluorescence detector is recycled to a cathode chamber of the electrolytic cell and used as electrolyte, and a signal is recorded and processed by a chromatography work station. The ion chromatography-electrochemical derivative-fluorescence analysis system has a single-pump single-flow path structure, an on-line oxidation mode and a rapid and sensitive method, and can be used for detecting and analyzing living beings and certain organic matters in medicine.

Description

technical field [0001] The invention relates to a chemical instrument analysis application system, in particular to an ion chromatography-on-line electrochemical derivation-fluorescence analysis system for detecting folic acid and methotrexate. Background technique [0002] Folic acid is one of the water-soluble B vitamins and plays a very important role in human metabolism. Methotrexate (methotrexate, MTX) is a folic acid homologue, which is mainly used to treat tumors and rheumatoid diseases. In the clinical treatment of rheumatoid diseases, patients take folic acid for detoxification 24 hours after taking methotrexate. Therefore, at the same time The detection of folic acid and methotrexate in serum is of great value in the diagnosis, treatment and prognosis of diseases. Common analysis methods for folic acid and methotrexate are high-performance liquid chromatography, electrochemical method, capillary electrophoresis, etc., usually separated by high-performance liquid c...

AI Technical Summary

Problems solved by technology

Common analysis methods for folic acid and methotrexate are high-performance liquid chromatography, electrochemical method, capillary electrophoresis, etc., usually separated by high-performance liquid chromatography, and analyzed by mass spectrometry as a detector. The instruments used in this method are expensive , the operating cost is high, and it is not suitable for popularization and operation

Folic acid substances have weak fluorescence properties. Through derivation, they can be transformed into pterin substances with strong fluorescence properties. Fluorescence method is used for detection. Common derivation methods include chemical derivation method, photochemical derivatization method and electrochemical derivation method. The chemical oxidation method needs to consume chemical reagents, which is time-consuming and laborious, and the photochemical oxidation method also requires a long reaction time

Existing technology uses high performance liquid chromatography-electrochemical derivation-fluorescence method to detect methotrexate, that is, the commercial electrochemical cell is placed at the injection valve position, and the sample is oxidized for 10 minutes when the sample is still, and then the column is switched to enter the fluorescence Detector detection, this method takes a long time and cannot truly achieve "on-line" oxidation. So far, there has been no report on the simultaneous determination of folic acid and methotrexate by ion chromatography-on-line electrochemical post-column derivatization-fluorescence method

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