Method for extracting anthocyanin from fruit dregs of Nitraria sibirica Pall

An extraction method and technology of Nitraria berries, applied in the direction of organic chemistry, etc., can solve the problems of not being utilized, and achieve the effect of lowering blood sugar, high extraction rate, and simple extraction method

Inactive Publication Date: 2011-05-18
QINGHAI QINGMEI BIOLOGICAL RESOURCES R&D
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of utilizing Nitraria japonica fruit juice and seeds, the pomace therein is discarded and has not been utilized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: Get 1000g Nitraria pomace, carry out enzymatic hydrolysis treatment, the enzyme preparation used contains cellulase, and the addition amount of cellulase is 0.15% of the weight of Nitraria pomace, enzymatic hydrolysis time 8min, temperature 20°C, and use sodium acetate to adjust the pH value in the enzymatic hydrolysis process to 3; add 30% ethanol to the material system after enzymatic hydrolysis for ultrasonic extraction, and the mass volume ratio (g:mL) of Nitraria pomace and ethanol is 1 : 10, extraction time 15min, temperature 45°C, ultrasonic power 150w, and in the ultrasonic extraction process, sodium acetate is used to maintain an acidic environment with a pH value of 3 in the system; Purify the anthocyanins in the centrifuged liquid with resin, the adsorption capacity of the resin is 50mg / g, the elution rate is 80%, and then eluted with 40% ethanol, and the extract and eluate are combined and collected at a temperature of 40 °C, under the conditio...

Embodiment 2

[0009] Embodiment 2: Get 1000g Nitraria pomace, carry out enzyme hydrolysis treatment, the enzyme preparation used is cellulase, and the addition amount of cellulase is 0.18% of the weight of Nitraria pomace, enzymatic hydrolysis time 10min, temperature 30 ℃, and use sodium acetate to adjust the pH value in the enzymatic hydrolysis process to be 4; add 35% ethanol to the material system after enzymatic hydrolysis for ultrasonic extraction, and the mass volume ratio (g:mL) of Nitraria pomace and ethanol is 1: 15. The extraction time is 20 minutes, the temperature is 50°C, and the ultrasonic power is 200w. During the ultrasonic extraction process, sodium acetate is used to maintain an acidic environment with a pH value of 4 in the system; the extract is centrifuged with a centrifuge and non-polar The resin is used to purify the anthocyanins in the liquid after centrifugation. The adsorption capacity of the resin is 60mg / g, and the elution rate is 70%. Then, it is eluted with 50% ...

Embodiment 3

[0010] Embodiment 3: Get 1000g Nitraria pomace, carry out enzymatic hydrolysis treatment, the enzyme preparation used contains cellulase, and the addition amount of cellulase is 0.2% of raw material weight, enzymatic hydrolysis time 14min, temperature 40 ℃, And use sodium acetate to regulate the pH value 4.6 in the enzymatic hydrolysis process; add 40% ethanol ultrasonic extraction in the material system after enzymatic hydrolysis, the mass volume ratio (g:mL) of Nitraria pomace and ethanol is 1:20, extraction time 25min, temperature 55°C, ultrasonic power 250w, and the ultrasonic extraction process uses sodium acetate to maintain an acidic environment with a pH value of 4.6 in the system; the extract is centrifuged with a centrifuge, and a non-polar resin is selected for centrifugation Purify the anthocyanins in the final liquid, the resin adsorption capacity is 70mg / g, and the elution rate is 90%, and then eluted with 60% ethanol, and the extract and eluate are combined and c...

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PUM

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Abstract

The invention relates to a method for extracting anthocyanin from the fruit dregs of Nitraria sibirica Pall. The method comprises the following working procedures of enzymolysis, extraction, separation, purification, elution, concentration and drying. The method in the invention is simple and the extraction rate is up to 80%, thus the resource can be utilized comprehensively; the method is beneficial for the industrialized production; and the method can provide a novel natural plant raw material of medicines and health food used for preventing tumor, reducing blood sugar, protecting the liver and soothing the gallbladder.

Description

1. Technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins from Nitraria pomace. 2. Background technology [0002] Nitraria L. is a small deciduous shrub of the genus Nitraria L. in the family Zygophyllaceae. It is a common wild saline-alkali plant in Northwest my country, which has high economic, ecological and medicinal value. The fruit is rich in vitamins, amino acids, polysaccharides and other nutritional active ingredients, especially amino acids, which are easily absorbed and utilized by the human body. The pomace contains an average of 0.37% anthocyanins. [0003] Anthocyanins are water-soluble pigments, which belong to flavonoids and mostly exist in the form of glycosides, also known as anthocyanins. As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have significant pharmacological effects. They have great application potential in food, cosmetics, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 山永凯李树志索有瑞任蓓蕾马宁安刘洪智杨莉华李兆千李安军年璟远张辉李宁
Owner QINGHAI QINGMEI BIOLOGICAL RESOURCES R&D
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