Nano-apatite/collagen compound stent and preparation method and application thereof
A composite scaffold and nano-calcium technology, applied in dental preparations, pharmaceutical formulations, dental prostheses, etc., can solve the problems of easy shedding, weak binding force between hydroxyapatite particles and collagen, and low biocompatibility. Not easy to fall off effect
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[0030] Hereinafter, the present invention will be further described in detail with reference to the examples and drawings, but the implementation of the present invention is not limited thereto.
[0031] The preparation method of the nano calcium phosphate / collagen composite scaffold of this embodiment is to firmly distribute the nano calcium phosphate salt in a collagen sponge with high porosity, and then form, freeze-dry, and cross-link it. It specifically includes the following steps and processes condition:
[0032] Collagen fiber and collagen are made in the laboratory and meet the standards.
[0033] 1. Disperse 2.0g of collagen fibers into 100ml of saturated calcium hydroxide solution, stir to fully swell the collagen fibers.
[0034] 2. Add 46ml of 0.03M sodium phosphate solution dropwise to the collagen fiber swelling material with a peristaltic pump at a rate of 0.1ml / min. The entire mineralization process is completed at room temperature.
[0035] 3. After mineralization, th...
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