Method for extracting ceramide

A technology of ceramide and extraction method, which is applied in the separation/purification of carboxylic acid amide, organic chemistry, etc., can solve the problems of not being suitable for large-scale production, etc., and achieve the effect of simple operation, high yield and high purity

Inactive Publication Date: 2011-06-15
天津强微特生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

However, the current main methods for extracting ceramides, such as high performance liquid chromatography and thin layer chromatography, are generally not suitable for large-scale production due to the constraints of experimental equipment and cost.

Method used

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Examples

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Embodiment 1

[0019] Soak fresh sea anemones in water, rinse them, put them outside to dry, and crush them with a blender. Weigh 500 g of broken sea anemones, add 500 ml of 95% industrial ethanol, and stir at 60° C. for 3 hours. Stop stirring, wait for the solid matter to settle down and collect the extract, repeat 3 times, combine the extract, filter with a 0.45 μm organic phase filter, then concentrate the extract with a rotary evaporator to obtain 15 grams of extract. Suspend the extract in 1L of distilled water, add 1L of ethyl acetate, stir for 4h, let stand for 2h to separate the layers, and extract the ethyl acetate layer. The extraction was repeated twice, the extracts were combined, and the ethyl acetate was evaporated to dryness under reduced pressure to obtain 6.2 g of a brown solid. This solid was dissolved in 10ml of dichloromethane, and put on a 10*180mm styrene-type CG161 macroporous resin column (Rohm and Haas), with a flow rate of 0.2ml / min. Wash the column with 20ml of n...

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Abstract

The invention provides a method for extracting ceramide from sea anemone, which comprises the following steps: crushing, extracting, leaching, adsorbing, eluting, drying by evaporation, recrystallizing and the like to obtain the high-purity ceramide. The method is simple to operate and easy for scale-up production.

Description

technical field [0001] The invention relates to a method for extracting ceramide, in particular to a method for extracting ceramide from sea anemones. Background technique [0002] Ceramide, namely N-fatty acyl sphingosine, is the basic unit of sphingomyelin, which is a component of cell membrane. It consists of a long chain of sphingosine and a saturated fatty acid connected to its amino terminal. It is an important class of biologically active substances in the body, mainly present in the cell membrane and intercellular matrix in the stratum corneum. [0003] Ceramide is the main component (>50%) of the intercellular liposomes of the outermost stratum corneum of human skin, and plays an important role in preventing moisture distribution, moisturizing and protecting the skin. The latest generation of ceramide moisturizers developed in recent years is a water-soluble lipid substance, which is similar in structure to the substance that makes up the stratum corneum of the ...

Claims

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Application Information

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IPC IPC(8): C07C233/20C07C231/24
Inventor 郑春阳张巍徐彬
Owner 天津强微特生物科技有限公司
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