Chemiluminescence quantitative detection kit for luteinizing hormone

A luteinizing hormone and chemiluminescence technology, which is applied in the field of luteinizing hormone chemiluminescence quantitative detection kits, can solve the problems of high price, difficult promotion, complicated operation, etc., and achieve the effect of stable detection ability and easy promotion

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign automatic chemiluminescent enzyme immunoassay analyzers and matching kits are mostly used in closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Preparation of LH quantitative detection kit

[0019] (1) Anti-LH antibody-coated plate preparation

[0020] a. Coating: Take 1mol / L Na 2 HPO 4 77.4ml and 1mol / L NaH 2 PO 4 Mix 22.6ml, add deionized water to 1000ml to form a 10-fold coating solution, dilute ten times before use, add an appropriate amount of LH monoclonal antibody (purchased from Meridianlifescience) and mix well, then add to the wells of the microplate , 100μl / well, 4°C for 16 hours;

[0021] b. Sealing: Discard the coating solution, pat dry on absorbent paper, add 3% BSA and 0.05% preservative (Proclin TM 300) of phosphate buffer (pH 7.4), 200 μl / well, 2 hours at 37°C;

[0022] c. Seal the bag: Discard the sealing solution, pat it dry on absorbent paper, pump it in a vacuum oven for 5 hours at room temperature, immediately vacuum seal the bag, check for air leakage, and re-seal the bag if there is a label. Store at 2-8°C.

[0023] (2) Preparation of enzyme markers

[0024] With...

Embodiment 2

[0037] Embodiment 2: the usage method of LH quantitative detection kit

[0038] 1. Reagent and Sample Preparation

[0039] (1) Reagent preparation

[0040] a. Equilibrate the kit at room temperature (18~26°C) for 20 minutes.

[0041] b. Take out the concentrated washing solution from the kit, dilute it 1:20 with fresh purified water and add it to the washing solution bottle of the plate washer.

[0042] (2) Sample preparation

[0043] Equilibrate the qualified serum or plasma to be tested at room temperature (18-26°C) for 20 minutes before use.

[0044] 2. Operation steps

[0045] a. Take out the required LH antibody-coated microwell plate and place it on the microwell rack

[0046] b. Add calibrator 1~6, quality control substance 1~2 and sample to be tested, 50 μl per well.

[0047] c. Add enzyme marker 100 μl / well.

[0048] d. Gently oscillate to mix.

[0049] e. Incubate at room temperature for 45 minutes.

[0050] f. Discard the waste liquid in the well.

[0051]...

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PUM

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Abstract

The invention discloses a chemiluminescence quantitative detection kit for luteinizing hormone. The kit comprises a luteinizing hormone detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a luteinizing hormone antibody. The kit can specifically and quantitatively detect the content of the luteinizing hormone in patient serum, and is used for the auxiliary diagnosis of diseases such as ovarian cyst, primary ovarian failure, dysfunction of hypophysis or hypothalamus, congenital testicular dysgenesis and the like. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and in particular relates to a chemiluminescent quantitative detection kit for luteinizing hormone (LH) and related preparation methods and usage methods. Background technique [0002] Luteinizing hormone (LH) is a hormone released by the hypothalamus in the human body, in the luteinizing hormone-releasing hormone (LH-RH, also known as gonadotropin-releasing hormone, Under the mediation of GnRH), it is synthesized by the pituitary gland and is also called interstitial cell stimulating hormone (ICSH) in males. LH is a glycoprotein with a molecular weight of about 30kD, which is composed of two different peptide chains - α and β subunits, which are connected by non-covalent bonds. The α-subunit of LH is similar in structure to the α-subunits of thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/543G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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