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Method for preparing quaternized chitosan and plasmid DNA compound particle loaded skin regeneration material

A quaternized chitosan, skin regeneration technology, applied in medical science, prosthesis, etc., can solve the problems of repeated operation of growth factors, instability of growth factors, easy degradation, etc., to reduce scar hyperplasia, good Histocompatibility, effect of promoting healing

Inactive Publication Date: 2011-07-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth factor is unstable and easy to degrade, and the direct injection of the growth factor requires repeated operations, which brings great inconvenience

Method used

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  • Method for preparing quaternized chitosan and plasmid DNA compound particle loaded skin regeneration material
  • Method for preparing quaternized chitosan and plasmid DNA compound particle loaded skin regeneration material
  • Method for preparing quaternized chitosan and plasmid DNA compound particle loaded skin regeneration material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0030] Dissolve quaternized chitosan in phosphate buffer to prepare 1 mg / mL quaternized chitosan solution, filter and sterilize through a 0.22 μm filter membrane; dissolve pDNA-PDGF in phosphate buffer Prepare a 0.1 mg / mL solution; mix the pDNA-PDGF solution and the quaternized chitosan solution in equal proportions, vibrate vigorously to mix evenly, and let stand to prepare a quaternized chitosan / pDNA-PDGF composite particle solution. Chitosan and collagen are prepared into a solution with a mass concentration of 0.1% by acetic acid solution with a mass concentration of 0.3%, and the mass ratio of chitosan and collagen is 5:5, and the collagen-chitosan blend is injected into the mold The collagen-chitosan three-dimensional porous scaffold was obtained by freeze-drying at -5°C, cross-linked with 0.1% glutaraldehyde for 48 hours, washed several times with triple distilled water, and freeze-dried at -5°C. α-octyl cyanoacrylate with good biocompatibility at 0.5 mg / cm 2 The amoun...

Embodiment 3

[0032] Dissolve quaternized chitosan in phosphate buffer to prepare 10mg / mL quaternized chitosan solution, filter and sterilize through a 0.22μm filter membrane; dissolve pDNA-aFGF in phosphate buffer Prepare a 2 mg / mL solution; mix the pDNA-aFGF solution and the quaternized chitosan solution in equal proportions, vibrate vigorously to mix evenly, and let stand to prepare a quaternized chitosan / pDNA-aFGF composite particle solution. Chitosan and collagen are prepared into a solution with a mass concentration of 0.5% by acetic acid solution with a mass concentration of 0.3%, and the mass ratio of chitosan and collagen is 2: 8, and the collagen-chitosan blend is injected into the mold The collagen-chitosan three-dimensional porous scaffold was obtained by freeze-drying at -20°C, cross-linked with 0.25% glutaraldehyde for 24 hours, washed several times with triple distilled water, and freeze-dried at -20°C. The silicone adhesive with good biocompatibility was added at 0.1mg / cm 2...

Embodiment 4

[0034] Dissolve quaternized chitosan in phosphate buffer to prepare 10mg / mL quaternized chitosan solution, filter and sterilize through a 0.22μm filter membrane; dissolve pDNA-bFGF in phosphate buffer Prepare a 2 mg / mL solution; mix the pDNA-bFGF solution and the quaternized chitosan solution in equal proportions, vibrate vigorously to mix evenly, and let stand to prepare a quaternized chitosan / pDNA-bFGF composite particle solution. Chitosan and collagen are prepared into a solution with a mass concentration of 1% by acetic acid solution with a mass concentration of 3%, and the mass ratio of chitosan and collagen is 1:9, and the collagen-chitosan blend is injected into the mold The collagen-chitosan three-dimensional porous scaffold was obtained by freeze-drying at -20°C, cross-linked with 0.5% glutaraldehyde for 24 hours, washed several times with triple distilled water, and freeze-dried at -20°C. The silicone adhesive with good biocompatibility was added at 0.2mg / cm 2 The a...

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Abstract

The invention discloses a method for preparing a quaternized chitosan and plasmid deoxyribonucleic acid (DNA) compound particle loaded skin regeneration material. The skin regeneration material is prepared by importing quaternized chitosan / plasmid DNA particles into a collagen-chitosan / silicone rubber double-layer scaffold. By the in-site transfection and expression of the imported quaternized chitosan / plasmid DNA particles on proangiogenic factors in the skin regeneration and repair process on skin defect wounds, the quick vascularization of the skin regeneration material on the wounds is promoted, the migration, proliferation and differentiation of wound cells are induced, and the wounds are quickly and well repaired. The skin regeneration material prepared by the method has quick vascularization performance and excellent wound repair ability, can be widely used for repairing and regenerating full-thickness skin defects such as wounds, burn and the like, and has better clinical application prospect.

Description

technical field [0001] The invention relates to a gene-enhanced skin regeneration material and a preparation method thereof, in particular to a collagen-chitosan / silicone rubber double-layer skin regeneration material of composite quaternized chitosan / plasmid DNA particles and a preparation method thereof . Background technique [0002] The skin is the largest organ of the human body, mainly composed of the epidermis, dermis and subcutaneous tissue, and is also rich in blood vessels and nerves. In daily life, due to various reasons such as trauma, burns, ulcers, surgery and congenital deformities, it is easy to cause skin defects. Although skin tissue has a strong regenerative ability, for large-area full-thickness skin defects, the regenerative ability of the skin is obviously insufficient due to the loss of the dermis. The key to treating full-thickness skin defects is to rebuild and regenerate dermal tissue, promote early wound closure, and avoid complications such as m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
Inventor 高长有马列毛峥伟郭瑞徐少骏
Owner ZHEJIANG UNIV
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